Kv11.1 channel subunit composition includes MinK and varies developmentally in mouse cardiac muscle

Xun Wang, Rongzuo Xu, Grant Abernathey, Jordan Taylor, M. B. Alzghoul, Kevin Hannon, Gregory H. Hockerman, Amber L. Pond

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The Kv11.1 (also ERG1) K+ channel underlies cardiac I Kr, a current that contributes to repolarization in mammalian heart. In mice, IKr current density decreases with development and studies suggest that changes in the structure and/or properties of the heteromultimeric IKr/Kv11.1 channel are responsible. Here, using immunohistochemistry, we report that total Kv11.1 α subunit protein is more abundant in neonatal heart and is distributed throughout both adult and neonatal ventricles with greater abundance in epicardia. Immunoblots reveal that the α subunit alternative splice variant, Kv11.1a, is more abundant in adult heart while the Kv11.1b variant is more abundant in neonatal heart. Additionally, MinK channel subunit protein is shown to co-assemble with Kv11.1 protein and is more abundant in neonatal heart. In summary, Kv11.1/IKr channel composition varies developmentally and the higher IKr current density in neonatal heart is likely attributable to higher abundance of Kv11.1/IKr channels, more specifically, the Kv11.1b splice variant.

Original languageEnglish (US)
Pages (from-to)2430-2437
Number of pages8
JournalDevelopmental Dynamics
Volume237
Issue number9
DOIs
StatePublished - Sep 2008

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Keywords

  • Cardiac ion channel
  • Kv11.1
  • Merg1a
  • MinK channel
  • Potassium channel

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    Wang, X., Xu, R., Abernathey, G., Taylor, J., Alzghoul, M. B., Hannon, K., Hockerman, G. H., & Pond, A. L. (2008). Kv11.1 channel subunit composition includes MinK and varies developmentally in mouse cardiac muscle. Developmental Dynamics, 237(9), 2430-2437. https://doi.org/10.1002/dvdy.21671