Kinetic flux profiling of nitrogen assimilation in Escherichia coli

Jie Yuan; William U. Fowler; Elizabeth Kimball; Wenyun Lu; Joshua D. Rabinowitz

doi:10.1038/nchembio816

Kinetic flux profiling of nitrogen assimilation in Escherichia coli

Jie Yuan
, William U. Fowler
, Elizabeth Kimball
, Wenyun Lu
, Joshua D. Rabinowitz

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

Original language	English (US)
Pages (from-to)	529-530
Number of pages	2
Journal	Nature Chemical Biology
Volume	2
Issue number	10
DOIs	https://doi.org/10.1038/nchembio816
State	Published - Oct 2006

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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10.1038/nchembio816

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title = "Kinetic flux profiling of nitrogen assimilation in Escherichia coli",

abstract = "We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.",

author = "Jie Yuan and Fowler, \{William U.\} and Elizabeth Kimball and Wenyun Lu and Rabinowitz, \{Joshua D.\}",

note = "Funding Information: We thank D. Botstein and N. Wingreen for their intellectual contributions and the US National Science Foundation, the National Institutes of Health and the Beckman Foundation for their financial support. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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AU - Kimball, Elizabeth

AU - Lu, Wenyun

AU - Rabinowitz, Joshua D.

N1 - Funding Information: We thank D. Botstein and N. Wingreen for their intellectual contributions and the US National Science Foundation, the National Institutes of Health and the Beckman Foundation for their financial support. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

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AB - We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

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Kinetic flux profiling of nitrogen assimilation in Escherichia coli

Abstract

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