We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.
|Original language||English (US)|
|Number of pages||2|
|Journal||Nature Chemical Biology|
|State||Published - Oct 2006|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology