Kinetic flux profiling of nitrogen assimilation in Escherichia coli

Jie Yuan, William U. Fowler, Elizabeth Kimball, Wenyun Lu, Joshua D. Rabinowitz

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

We present a new method for probing cellular metabolic fluxes that is based on the kinetics of assimilation of isotope-labeled nutrient into a diversity of downstream metabolites. In the case of nitrogen assimilation, half-maximal labeling of most metabolites occurs in 10-300 s. Fluxes measured on the basis of the kinetics of nitrogen assimilation in exponentially growing E. coli agree well with those fluxes predicted to allow optimal biomass production.

Original languageEnglish (US)
Pages (from-to)529-530
Number of pages2
JournalNature Chemical Biology
Volume2
Issue number10
DOIs
StatePublished - Oct 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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