Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein

Neel H. Shah; Miquel Vila-Perelló; Tom W. Muir

doi:10.1002/anie.201102909

Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein

Neel H. Shah
, Miquel Vila-Perelló
, Tom W. Muir

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

Charge-swapping of key intermolecular ion clusters in the naturally split DnaE intein from Nostoc punctiforme (Npu_WT) alters split intein binding affinities and trans-splicing kinetics. This concept was used to engineer the new split intein Npu_MUT with low cross-reactivity to Npu_WT. This intein pair can be used to catalyze multiple trans-splicing reactions in one pot with kinetically controlled selectivity (see picture).

Original language	English (US)
Pages (from-to)	6511-6515
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	29
DOIs	https://doi.org/10.1002/anie.201102909
State	Published - Jul 11 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

Keywords

ion pairs
protein design
protein-protein interactions
semisynthesis

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10.1002/anie.201102909

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abstract = "Charge-swapping of key intermolecular ion clusters in the naturally split DnaE intein from Nostoc punctiforme (NpuWT) alters split intein binding affinities and trans-splicing kinetics. This concept was used to engineer the new split intein NpuMUT with low cross-reactivity to NpuWT. This intein pair can be used to catalyze multiple trans-splicing reactions in one pot with kinetically controlled selectivity (see picture).",

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AU - Shah, Neel H.

AU - Vila-Perelló, Miquel

AU - Muir, Tom W.

PY - 2011/7/11

Y1 - 2011/7/11

N2 - Charge-swapping of key intermolecular ion clusters in the naturally split DnaE intein from Nostoc punctiforme (NpuWT) alters split intein binding affinities and trans-splicing kinetics. This concept was used to engineer the new split intein NpuMUT with low cross-reactivity to NpuWT. This intein pair can be used to catalyze multiple trans-splicing reactions in one pot with kinetically controlled selectivity (see picture).

AB - Charge-swapping of key intermolecular ion clusters in the naturally split DnaE intein from Nostoc punctiforme (NpuWT) alters split intein binding affinities and trans-splicing kinetics. This concept was used to engineer the new split intein NpuMUT with low cross-reactivity to NpuWT. This intein pair can be used to catalyze multiple trans-splicing reactions in one pot with kinetically controlled selectivity (see picture).

KW - ion pairs

KW - protein design

KW - protein-protein interactions

KW - semisynthesis

UR - https://www.scopus.com/pages/publications/79959981826

UR - https://www.scopus.com/pages/publications/79959981826#tab=citedBy

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 29

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Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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