Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein

Neel H. Shah, Miquel Vila-Perelló, Tom W. Muir

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Charge-swapping of key intermolecular ion clusters in the naturally split DnaE intein from Nostoc punctiforme (NpuWT) alters split intein binding affinities and trans-splicing kinetics. This concept was used to engineer the new split intein NpuMUT with low cross-reactivity to NpuWT. This intein pair can be used to catalyze multiple trans-splicing reactions in one pot with kinetically controlled selectivity (see picture).

Original languageEnglish (US)
Pages (from-to)6511-6515
Number of pages5
JournalAngewandte Chemie - International Edition
Volume50
Issue number29
DOIs
StatePublished - Jul 11 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • ion pairs
  • protein design
  • protein-protein interactions
  • semisynthesis

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