@article{afcc1648c188444eb1757ce3b2602f62,
title = "JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila",
abstract = "In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1{\textquoteright}s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.",
author = "Christian Albig and Chao Wang and Dann, {Geoffrey P.} and Felix Wojcik and Tam{\'a}s Schauer and Silke Krause and Sylvain Maenner and Weili Cai and Yeran Li and Jack Girton and Muir, {Tom W.} and J{\o}rgen Johansen and Johansen, {Kristen M.} and Becker, {Peter B.} and Catherine Regnard",
note = "Funding Information: We thank A. Lukacs, K. Prayitno, and L. Harpprecht for sharing embryo extracts. We thank A. Scacchetti for establishing the D. virilis spike-in approach. We thank B.V. Andrianov to kindly share D. virilis cell line and J. Kadonaga for NDF reagents. We thank A. Imhof, I. Forn{\'e}, and M. Wirth at ZFP for mass spectrometry services, S. Krebs and the LAFUGA Genomics Facility for next generation sequencing, T. Straub and the Bioinformatics Unit for providing the high performance computational cluster, E. Kremmer for monoclonal antibody generation, H. Loyd for help with antibody labelings of polytene chromosomes and C. Grimaud for confocal imaging. This work was supported by a grant from the Deutsche Forschungsgemeinschaft to PBB (Be1140/8-1). C.A. acknowledges a DFG fellowship from the Graduate School for Quantitative Biosciences Munich (QBM). Work in the K.M.J. and J.J. laboratory was supported by NIH grant R01 GM62916 and the Roy J. Carver Foundation. National Institutes of Health (NIH) Grants R37 GM086868, R01 GM107047, and P01 CA196539 supported the research in the laboratory of T.W.M. F.W. was funded by a postdoctoral fellowship from the German Research Foundation (WO 2039/1-1). Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41467-019-13174-6",
language = "English (US)",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}