TY - JOUR
T1 - Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development
AU - Christiaen, Lionel
AU - Wagner, Eileen
AU - Shi, Weiyang
AU - Levine, Michael
PY - 2009
Y1 - 2009
N2 - This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trate amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.
AB - This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trate amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.
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U2 - 10.1101/pdb.prot5344
DO - 10.1101/pdb.prot5344
M3 - Article
C2 - 20150091
AN - SCOPUS:72149087973
SN - 1940-3402
VL - 4
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 12
ER -