Isolation of novel herpes simplex virus type 1 derivatives with tandem duplications of DNA sequences encoding immediate-early mRNA-5 and an origin of replication

K. Umene, L. W. Enquist

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Two naturally occurring variations of herpes simplex virus type 1 (Patton strain) with novel tandem DNA sequence duplications in the S component were isolated, and the DNA was characterized. These variants were identified among a number of plaque isolates by the appearance of new restriction enzyme fragments that hybridized with radiolabeled DNA from the BamHI Z fragment (map coordinates 0.936 to 0.949) located in the unique S region. One isolate, SP26-3, carried a 3.1-kilobase-pair duplication defined by recombination between a site in the BamHI Z fragment and a site near the origin of replicated in the inverted repeat sequence of the S component carried by the EcoRI H fragment. The other isolate, SP22-4, carried a 3.5-kilobase-pair duplication defined by a recombination event between a tandem repeat array in the BamHI Z fragment and a site near the amino terminus of the Vmw175 gene in the S-region inverted repeat sequence contained in the Eco RI K fragment. Both duplicated segments contained the entire immediate early mRNA-5 coding region as well as the origin of replication location in the inverted repeat sequence of the S component. The DNA sequence of each duplication joint was determined.

Original languageEnglish (US)
Pages (from-to)607-615
Number of pages9
JournalJournal of virology
Volume53
Issue number2
DOIs
StatePublished - 1985

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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