TY - JOUR
T1 - Isolation of mutations in the α operon of Escherichia coli that suppress the transcriptional defect conferred by a mutation in the porin regulatory gene envZ
AU - Garrett, S.
AU - Silhavy, T. J.
PY - 1987
Y1 - 1987
N2 - One class of mutations in the envZ gene of Escherichia coli K-12 confers a pleiotropic defect on the expression of several genes, including ompF, lamB, and phoA, that are otherwise not commonly regulated. Four second-site mutations that suppress this transcriptional defect have been isolated by using a procedure that circumvented the problem of intragenic suppressors, including true revertants. All four mutations have been mapped to the genes of the α operon and have been assigned tentatively to the gene rpoA, which specifies the α subunit of RNA polymerase. The mutations, referred to as sez (for suppressor of envZ), did not appear to confer a phenotype on an otherwise wild-type strain and did not suppress the transcriptional defects conferred by several other phenotypic classes of envZ mutations, including amber mutations. Our results led us to postulate that the α subunit or some other component of the α operon plays a role in determining the specificity of gene expression.
AB - One class of mutations in the envZ gene of Escherichia coli K-12 confers a pleiotropic defect on the expression of several genes, including ompF, lamB, and phoA, that are otherwise not commonly regulated. Four second-site mutations that suppress this transcriptional defect have been isolated by using a procedure that circumvented the problem of intragenic suppressors, including true revertants. All four mutations have been mapped to the genes of the α operon and have been assigned tentatively to the gene rpoA, which specifies the α subunit of RNA polymerase. The mutations, referred to as sez (for suppressor of envZ), did not appear to confer a phenotype on an otherwise wild-type strain and did not suppress the transcriptional defects conferred by several other phenotypic classes of envZ mutations, including amber mutations. Our results led us to postulate that the α subunit or some other component of the α operon plays a role in determining the specificity of gene expression.
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U2 - 10.1128/jb.169.4.1379-1385.1987
DO - 10.1128/jb.169.4.1379-1385.1987
M3 - Article
C2 - 3031009
AN - SCOPUS:0023185602
SN - 0021-9193
VL - 169
SP - 1379
EP - 1385
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 4
ER -