TY - JOUR
T1 - Isolation of individual cells and tissues from electroporated sea squirt (Ciona) embryos by fluorescence-activated cell sorting (FACS)
AU - Christiaen, Lionel
AU - Wagner, Eileen
AU - Shi, Weiyang
AU - Levine, Michael
PY - 2009
Y1 - 2009
N2 - This protocol describes the dissociation of electroporated sea squirt (Ciona) embryos and the subsequent isolation of individual cells, cell lineages, and tissues, using fluorescence-activated cell sorting (FACS). Sorted samples can be used for RNA extraction and reverse transcriptase polymerase chain reaction (RT-PCR) or microarray analysis. Alternatively, dissociated samples can be used for the subsequent culturing and/or chemical treatment of isolated embryonic cells. This method permits the identification of effector genes responsible for changes in cell shape, polarity, or motility in a given tissue.
AB - This protocol describes the dissociation of electroporated sea squirt (Ciona) embryos and the subsequent isolation of individual cells, cell lineages, and tissues, using fluorescence-activated cell sorting (FACS). Sorted samples can be used for RNA extraction and reverse transcriptase polymerase chain reaction (RT-PCR) or microarray analysis. Alternatively, dissociated samples can be used for the subsequent culturing and/or chemical treatment of isolated embryonic cells. This method permits the identification of effector genes responsible for changes in cell shape, polarity, or motility in a given tissue.
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U2 - 10.1101/pdb.prot5349
DO - 10.1101/pdb.prot5349
M3 - Article
C2 - 20150096
AN - SCOPUS:72149110852
SN - 1940-3402
VL - 4
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 12
ER -