Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells

Nicholas Jones, Thomas Shenk

Research output: Contribution to journalArticle

460 Scopus citations

Abstract

A series of adenovirus type 5 (Ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. The mutants were selected as variants which lack the Xba I endonuclease cleavage site at 4 map units on the viral chromosome. The deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. The mutants can be propagated in 293 cells (Ad5-transformed human embryonic kidney cells), but are defective for growth in HeLa or human embryonic kidney cells. No viral DNA synthesis was observed in mutant virus-infected HeLa cells. All but one of the deletion mutants tested were defective for transformation of rat embryo and rat embryo brain cells.

Original languageEnglish (US)
Pages (from-to)683-689
Number of pages7
JournalCell
Volume17
Issue number3
DOIs
StatePublished - Jul 1979
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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