Isolation and characterization of mutants deleted for the sulA-ompA region of the Escherichia coli K-12 chromosome

Erhard Bremer; Thomas J. Silhavy; Marga Maldener; Stewart T. Cole

doi:10.1111/j.1574-6968.1986.tb01266.x

Isolation and characterization of mutants deleted for the sulA-ompA region of the Escherichia coli K-12 chromosome

Erhard Bremer, Thomas J. Silhavy, Marga Maldener, Stewart T. Cole

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We have isolated mutants deleted for different segments of the sulA-ompA region of the Escherichia coli K-12 chromosome using gene fusion techniques. Genetic and physical analysis showed that the deletions ranged from 500 to more than 4000 base pairs (bp) Strains were found in which all, or part, of the sulA and ompA genes had been deleted.

Original language	English (US)
Pages (from-to)	173-178
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	33
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1986.tb01266.x
State	Published - Feb 1986
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

Keywords

Outer membrane protein
SOS
gene fusion
phage lambda

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AU - Silhavy, Thomas J.

AU - Maldener, Marga

AU - Cole, Stewart T.

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AB - We have isolated mutants deleted for different segments of the sulA-ompA region of the Escherichia coli K-12 chromosome using gene fusion techniques. Genetic and physical analysis showed that the deletions ranged from 500 to more than 4000 base pairs (bp) Strains were found in which all, or part, of the sulA and ompA genes had been deleted.

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