Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

M. F. DeFlaun; J. K. Fredrickson; H. Dong; S. M. Pfiffner; Tullis C. Onstott; D. L. Balkwill; S. H. Streger; E. Stackebrandt; S. Knoessen; E. van Heerden

doi:10.1016/j.syapm.2006.04.003

Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

M. F. DeFlaun
, J. K. Fredrickson
, H. Dong
, S. M. Pfiffner
, Tullis C. Onstott
, D. L. Balkwill
, S. H. Streger
, E. Stackebrandt
, S. Knoessen
, E. van Heerden

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O₂, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366^T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

Original language	English (US)
Pages (from-to)	152-164
Number of pages	13
Journal	Systematic and Applied Microbiology
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/j.syapm.2006.04.003
State	Published - Mar 8 2007

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Ecology, Evolution, Behavior and Systematics
Microbiology

Keywords

Deep subsurface bacteria
Extremophiles
Geobacillus thermoleovorans
Lipase
Thermophilic bacilli

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10.1016/j.syapm.2006.04.003

Cite this

DeFlaun, M. F., Fredrickson, J. K., Dong, H., Pfiffner, S. M., Onstott, T. C., Balkwill, D. L., Streger, S. H., Stackebrandt, E., Knoessen, S., & van Heerden, E. (2007). Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine. Systematic and Applied Microbiology, 30(2), 152-164. https://doi.org/10.1016/j.syapm.2006.04.003

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title = "Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine",

abstract = "A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6\%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.",

keywords = "Deep subsurface bacteria, Extremophiles, Geobacillus thermoleovorans, Lipase, Thermophilic bacilli",

author = "DeFlaun, \{M. F.\} and Fredrickson, \{J. K.\} and H. Dong and Pfiffner, \{S. M.\} and Onstott, \{Tullis C.\} and Balkwill, \{D. L.\} and Streger, \{S. H.\} and E. Stackebrandt and S. Knoessen and \{van Heerden\}, E.",

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DeFlaun, MF, Fredrickson, JK, Dong, H, Pfiffner, SM, Onstott, TC, Balkwill, DL, Streger, SH, Stackebrandt, E, Knoessen, S & van Heerden, E 2007, 'Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine', Systematic and Applied Microbiology, vol. 30, no. 2, pp. 152-164. https://doi.org/10.1016/j.syapm.2006.04.003

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AU - DeFlaun, M. F.

AU - Fredrickson, J. K.

AU - Dong, H.

AU - Pfiffner, S. M.

AU - Onstott, Tullis C.

AU - Balkwill, D. L.

AU - Streger, S. H.

AU - Stackebrandt, E.

AU - Knoessen, S.

AU - van Heerden, E.

PY - 2007/3/8

Y1 - 2007/3/8

N2 - A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

AB - A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

KW - Deep subsurface bacteria

KW - Extremophiles

KW - Geobacillus thermoleovorans

KW - Lipase

KW - Thermophilic bacilli

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Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

Abstract

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Keywords

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