Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

M. F. DeFlaun, J. K. Fredrickson, H. Dong, S. M. Pfiffner, Tullis C. Onstott, D. L. Balkwill, S. H. Streger, E. Stackebrandt, S. Knoessen, E. van Heerden

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32 Scopus citations

Abstract

A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

Original languageEnglish (US)
Pages (from-to)152-164
Number of pages13
JournalSystematic and Applied Microbiology
Volume30
Issue number2
DOIs
StatePublished - Mar 8 2007

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Keywords

  • Deep subsurface bacteria
  • Extremophiles
  • Geobacillus thermoleovorans
  • Lipase
  • Thermophilic bacilli

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    DeFlaun, M. F., Fredrickson, J. K., Dong, H., Pfiffner, S. M., Onstott, T. C., Balkwill, D. L., Streger, S. H., Stackebrandt, E., Knoessen, S., & van Heerden, E. (2007). Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine. Systematic and Applied Microbiology, 30(2), 152-164. https://doi.org/10.1016/j.syapm.2006.04.003