Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

M. F. DeFlaun; J. K. Fredrickson; H. Dong; S. M. Pfiffner; Tullis C. Onstott; D. L. Balkwill; S. H. Streger; E. Stackebrandt; S. Knoessen; E. van Heerden

doi:10.1016/j.syapm.2006.04.003

Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

M. F. DeFlaun, J. K. Fredrickson, H. Dong, S. M. Pfiffner, Tullis C. Onstott, D. L. Balkwill, S. H. Streger, E. Stackebrandt, S. Knoessen, E. van Heerden

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39 Scopus citations

Abstract

A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O₂, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366^T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

Original language	English (US)
Pages (from-to)	152-164
Number of pages	13
Journal	Systematic and Applied Microbiology
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/j.syapm.2006.04.003
State	Published - Mar 8 2007

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Ecology, Evolution, Behavior and Systematics
Microbiology

Keywords

Deep subsurface bacteria
Extremophiles
Geobacillus thermoleovorans
Lipase
Thermophilic bacilli

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10.1016/j.syapm.2006.04.003

Cite this

DeFlaun, M. F., Fredrickson, J. K., Dong, H., Pfiffner, S. M., Onstott, T. C., Balkwill, D. L., Streger, S. H., Stackebrandt, E., Knoessen, S., & van Heerden, E. (2007). Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine. Systematic and Applied Microbiology, 30(2), 152-164. https://doi.org/10.1016/j.syapm.2006.04.003

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title = "Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine",

abstract = "A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.",

keywords = "Deep subsurface bacteria, Extremophiles, Geobacillus thermoleovorans, Lipase, Thermophilic bacilli",

author = "DeFlaun, {M. F.} and Fredrickson, {J. K.} and H. Dong and Pfiffner, {S. M.} and Onstott, {Tullis C.} and Balkwill, {D. L.} and Streger, {S. H.} and E. Stackebrandt and S. Knoessen and {van Heerden}, E.",

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DeFlaun, MF, Fredrickson, JK, Dong, H, Pfiffner, SM, Onstott, TC, Balkwill, DL, Streger, SH, Stackebrandt, E, Knoessen, S & van Heerden, E 2007, 'Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine', Systematic and Applied Microbiology, vol. 30, no. 2, pp. 152-164. https://doi.org/10.1016/j.syapm.2006.04.003

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AU - Fredrickson, J. K.

AU - Dong, H.

AU - Pfiffner, S. M.

AU - Onstott, Tullis C.

AU - Balkwill, D. L.

AU - Streger, S. H.

AU - Stackebrandt, E.

AU - Knoessen, S.

AU - van Heerden, E.

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N2 - A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

AB - A thermophilic facultative bacterial isolate was recovered from 3.2 km depth in a gold mine in South Africa. This isolate, designated GE-7, was cultivated from pH 8.0, 50 °C water from a dripping fracture near the top of an exploration tunnel. GE-7 grows optimally at 65 °C and pH 6.5 on a wide range of carbon substrates including cellobiose, hydrocarbons and lactate. In addition to O2, GE-7 also utilizes nitrate as an electron acceptor. GE-7 is a long rod-shaped bacterium (4-6 μm long×0.5 μm wide) with terminal endospores and flagella. Phylogenetic analysis of GE-7 16S rDNA sequence revealed high sequence similarity with G. thermoleovorans DSM 5366T (99.6%), however, certain phenotypic characteristics of GE-7 were distinct from this and other previously described strains of G. thermoleovorans.

KW - Deep subsurface bacteria

KW - Extremophiles

KW - Geobacillus thermoleovorans

KW - Lipase

KW - Thermophilic bacilli

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Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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