Abstract
The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.
Original language | English (US) |
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Pages (from-to) | 753-763 |
Number of pages | 11 |
Journal | Biophysical Journal |
Volume | 15 |
Issue number | 8 |
DOIs | |
State | Published - 1975 |
All Science Journal Classification (ASJC) codes
- Biophysics