Iontophoresis of cyclic AMP

M. H. Cohen, D. J. Drage, A. Robertson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.

Original languageEnglish (US)
Pages (from-to)753-763
Number of pages11
JournalBiophysical Journal
Volume15
Issue number8
DOIs
StatePublished - 1975

All Science Journal Classification (ASJC) codes

  • Biophysics

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