Iontophoresis of cyclic AMP

M. H. Cohen; D. J. Drage; A. Robertson

doi:10.1016/S0006-3495(75)85852-8

Iontophoresis of cyclic AMP

M. H. Cohen, D. J. Drage, A. Robertson

Chemistry

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.

Original language	English (US)
Pages (from-to)	753-763
Number of pages	11
Journal	Biophysical Journal
Volume	15
Issue number	8
DOIs	https://doi.org/10.1016/S0006-3495(75)85852-8
State	Published - 1975

All Science Journal Classification (ASJC) codes

Biophysics

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10.1016/S0006-3495(75)85852-8

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title = "Iontophoresis of cyclic AMP",

abstract = "The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.",

author = "Cohen, {M. H.} and Drage, {D. J.} and A. Robertson",

note = "Funding Information: A. Robertson is an Alfred P. Sloan Foundation Fellow, 1973-75. This work was supported by National Science Foundation grantGB-30784 tothe University ofChicago. Receivedforpublication6August 1974 andin revisedfonn21 April 1975.",

year = "1975",

doi = "10.1016/S0006-3495(75)85852-8",

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volume = "15",

pages = "753--763",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Biophysical Society",

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T1 - Iontophoresis of cyclic AMP

AU - Cohen, M. H.

AU - Drage, D. J.

AU - Robertson, A.

N1 - Funding Information: A. Robertson is an Alfred P. Sloan Foundation Fellow, 1973-75. This work was supported by National Science Foundation grantGB-30784 tothe University ofChicago. Receivedforpublication6August 1974 andin revisedfonn21 April 1975.

PY - 1975

Y1 - 1975

N2 - The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.

AB - The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.

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U2 - 10.1016/S0006-3495(75)85852-8

DO - 10.1016/S0006-3495(75)85852-8

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AN - SCOPUS:0016773108

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VL - 15

SP - 753

EP - 763

JO - Biophysical Journal

JF - Biophysical Journal

IS - 8

ER -

Iontophoresis of cyclic AMP

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