TY - GEN
T1 - Inverse flash nanoprecipitation for biologics encapsulation
T2 - Understanding process losses via an extraction protocol
AU - Markwalter, Chester E.
AU - Prud'Homme, Robert K.
N1 - Publisher Copyright:
© 2018 American Chemical Society. All rights reserved.
PY - 2017
Y1 - 2017
N2 - Inverse Flash NanoPrecipitation (iFNP) is a scalable technique for encapsulating hydrophilic molecules such as peptides and proteins in nanoparticles at high loadings. These nanoparticles, which have a hydrophilic core and hydrophobic corona, may be incorporated directly into microparticles for sustained release applications. Use of iFNP instead of the traditional double emulsion process can enable higher loadings and encapsulation efficiencies. We have developed an extraction-based protocol to rapidly evaluate the impact of iFNP formulation parameters on process losses without the added complexity of producing microparticles. Among other parameters, the external osmotic pressure and, for larger biologics, the crosslinking density were found to impact the extraction process. Microparticles were subsequently produced with a target loading of 25% of a model biologic at greater than 90% encapsulation efficiency using these insights.
AB - Inverse Flash NanoPrecipitation (iFNP) is a scalable technique for encapsulating hydrophilic molecules such as peptides and proteins in nanoparticles at high loadings. These nanoparticles, which have a hydrophilic core and hydrophobic corona, may be incorporated directly into microparticles for sustained release applications. Use of iFNP instead of the traditional double emulsion process can enable higher loadings and encapsulation efficiencies. We have developed an extraction-based protocol to rapidly evaluate the impact of iFNP formulation parameters on process losses without the added complexity of producing microparticles. Among other parameters, the external osmotic pressure and, for larger biologics, the crosslinking density were found to impact the extraction process. Microparticles were subsequently produced with a target loading of 25% of a model biologic at greater than 90% encapsulation efficiency using these insights.
UR - http://www.scopus.com/inward/record.url?scp=85051223642&partnerID=8YFLogxK
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U2 - 10.1021/bk-2017-1271.ch012
DO - 10.1021/bk-2017-1271.ch012
M3 - Conference contribution
AN - SCOPUS:85051223642
SN - 9780841232747
T3 - ACS Symposium Series
SP - 275
EP - 296
BT - Control of Amphiphile Self-Assembling at the Molecular Level
A2 - Ilies, Marc A.
PB - American Chemical Society
ER -