Inverse flash nanoprecipitation for biologics encapsulation: Understanding process losses via an extraction protocol

Chester E. Markwalter, Robert K. Prud'Homme

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

Inverse Flash NanoPrecipitation (iFNP) is a scalable technique for encapsulating hydrophilic molecules such as peptides and proteins in nanoparticles at high loadings. These nanoparticles, which have a hydrophilic core and hydrophobic corona, may be incorporated directly into microparticles for sustained release applications. Use of iFNP instead of the traditional double emulsion process can enable higher loadings and encapsulation efficiencies. We have developed an extraction-based protocol to rapidly evaluate the impact of iFNP formulation parameters on process losses without the added complexity of producing microparticles. Among other parameters, the external osmotic pressure and, for larger biologics, the crosslinking density were found to impact the extraction process. Microparticles were subsequently produced with a target loading of 25% of a model biologic at greater than 90% encapsulation efficiency using these insights.

Original languageEnglish (US)
Title of host publicationControl of Amphiphile Self-Assembling at the Molecular Level
Subtitle of host publicationSupra-Molecular Assemblies with Tuned Physicochemical Properties for Delivery Applications
EditorsMarc A. Ilies
PublisherAmerican Chemical Society
Pages275-296
Number of pages22
ISBN (Print)9780841232747
DOIs
StatePublished - Jan 1 2017

Publication series

NameACS Symposium Series
Volume1271
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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    Markwalter, C. E., & Prud'Homme, R. K. (2017). Inverse flash nanoprecipitation for biologics encapsulation: Understanding process losses via an extraction protocol. In M. A. Ilies (Ed.), Control of Amphiphile Self-Assembling at the Molecular Level: Supra-Molecular Assemblies with Tuned Physicochemical Properties for Delivery Applications (pp. 275-296). (ACS Symposium Series; Vol. 1271). American Chemical Society. https://doi.org/10.1021/bk-2017-1271.ch012