Intramolecular rearrangements as a consequence of the dephosphorylation of phosphoaspartate residues in proteins

Scott Napper; Peter M. Wolanin; Daniel J. Webre; Jason Kindrachuk; Bruce Waygood; Jeffry B. Stock

doi:10.1016/S0014-5793(03)00132-7

Intramolecular rearrangements as a consequence of the dephosphorylation of phosphoaspartate residues in proteins

Scott Napper
, Peter M. Wolanin
, Daniel J. Webre
, Jason Kindrachuk
, Bruce Waygood
, Jeffry B. Stock

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Aspartate phosphorylation induces changes in protein conformation that are used to regulate processes ranging from gene expression and cell differentiation to cell motility and the generation of electrochemical gradients across membranes. We show here that dephosphorylation of the phosphoaspartate in the chemotaxis response regulator CheY can result in the loss of a water molecule that may be due to formation of a succinimide intermediate.

Original language	English (US)
Pages (from-to)	77-80
Number of pages	4
Journal	FEBS Letters
Volume	538
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00132-7
State	Published - Mar 13 2003

All Science Journal Classification (ASJC) codes

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry
Cell Biology

Keywords

CheY
OmpR
Phosphoaspartate
Response regulator
Succinimide

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10.1016/S0014-5793(03)00132-7

Cite this

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title = "Intramolecular rearrangements as a consequence of the dephosphorylation of phosphoaspartate residues in proteins",

abstract = "Aspartate phosphorylation induces changes in protein conformation that are used to regulate processes ranging from gene expression and cell differentiation to cell motility and the generation of electrochemical gradients across membranes. We show here that dephosphorylation of the phosphoaspartate in the chemotaxis response regulator CheY can result in the loss of a water molecule that may be due to formation of a succinimide intermediate.",

keywords = "CheY, OmpR, Phosphoaspartate, Response regulator, Succinimide",

author = "Scott Napper and Wolanin, \{Peter M.\} and Webre, \{Daniel J.\} and Jason Kindrachuk and Bruce Waygood and Stock, \{Jeffry B.\}",

note = "Funding Information: We gratefully acknowledge D. Little and S. Kyin for acquisition of HPLC/MS data and helpful discussions, and M.N. Levit for technical advice and assistance with figures. This work was supported in part by a grant from the National Sciences and Engineering Research Council of Canada to S.N., a grant from the Defense Advanced Research Projects Agency (No. 3416183) to J.B.S., and by NIH Fellowship F32 GM064228 to P.M.W. ",

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doi = "10.1016/S0014-5793(03)00132-7",

language = "English (US)",

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pages = "77--80",

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T1 - Intramolecular rearrangements as a consequence of the dephosphorylation of phosphoaspartate residues in proteins

AU - Napper, Scott

AU - Wolanin, Peter M.

AU - Webre, Daniel J.

AU - Kindrachuk, Jason

AU - Waygood, Bruce

AU - Stock, Jeffry B.

N1 - Funding Information: We gratefully acknowledge D. Little and S. Kyin for acquisition of HPLC/MS data and helpful discussions, and M.N. Levit for technical advice and assistance with figures. This work was supported in part by a grant from the National Sciences and Engineering Research Council of Canada to S.N., a grant from the Defense Advanced Research Projects Agency (No. 3416183) to J.B.S., and by NIH Fellowship F32 GM064228 to P.M.W.

PY - 2003/3/13

Y1 - 2003/3/13

N2 - Aspartate phosphorylation induces changes in protein conformation that are used to regulate processes ranging from gene expression and cell differentiation to cell motility and the generation of electrochemical gradients across membranes. We show here that dephosphorylation of the phosphoaspartate in the chemotaxis response regulator CheY can result in the loss of a water molecule that may be due to formation of a succinimide intermediate.

AB - Aspartate phosphorylation induces changes in protein conformation that are used to regulate processes ranging from gene expression and cell differentiation to cell motility and the generation of electrochemical gradients across membranes. We show here that dephosphorylation of the phosphoaspartate in the chemotaxis response regulator CheY can result in the loss of a water molecule that may be due to formation of a succinimide intermediate.

KW - CheY

KW - OmpR

KW - Phosphoaspartate

KW - Response regulator

KW - Succinimide

UR - https://www.scopus.com/pages/publications/0037434838

UR - https://www.scopus.com/pages/publications/0037434838#tab=citedBy

U2 - 10.1016/S0014-5793(03)00132-7

DO - 10.1016/S0014-5793(03)00132-7

M3 - Article

C2 - 12633856

AN - SCOPUS:0037434838

SN - 0014-5793

VL - 538

SP - 77

EP - 80

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Intramolecular rearrangements as a consequence of the dephosphorylation of phosphoaspartate residues in proteins

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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