Intracellular calcium release in N1E-115 neuroblastoma cells is mediated by the M1 muscarinic receptor subtype and is antagonized by McN-A-343

Chris Mathes; Samuel S.H. Wang; Hugo M. Vargas; Stuart H. Thompson

doi:10.1016/0006-8993(92)91225-4

Intracellular calcium release in N1E-115 neuroblastoma cells is mediated by the M1 muscarinic receptor subtype and is antagonized by McN-A-343

Chris Mathes, Samuel S.H. Wang, Hugo M. Vargas, Stuart H. Thompson

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (±)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (±)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC₅₀ of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.

Original language	English (US)
Pages (from-to)	307-310
Number of pages	4
Journal	Brain Research
Volume	585
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(92)91225-4
State	Published - Jul 10 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

Keywords

(±)BN228 (4-[N]-(chlorophenyl)carboxyloxy-pent-2-ynyltrimethylammonium Br)
4-DAMP
AFDX116
Calcium release
McN-A-343
Muscarinic receptor subtype
N1E-115 neuroblastoma
Pirenzepine

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10.1016/0006-8993(92)91225-4

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title = "Intracellular calcium release in N1E-115 neuroblastoma cells is mediated by the M1 muscarinic receptor subtype and is antagonized by McN-A-343",

abstract = "Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (±)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (±)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC50 of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.",

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author = "Chris Mathes and Wang, {Samuel S.H.} and Vargas, {Hugo M.} and Thompson, {Stuart H.}",

year = "1992",

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T1 - Intracellular calcium release in N1E-115 neuroblastoma cells is mediated by the M1 muscarinic receptor subtype and is antagonized by McN-A-343

AU - Mathes, Chris

AU - Wang, Samuel S.H.

AU - Vargas, Hugo M.

AU - Thompson, Stuart H.

PY - 1992/7/10

Y1 - 1992/7/10

N2 - Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (±)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (±)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC50 of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.

AB - Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (±)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (±)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC50 of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.

KW - (±)BN228 (4-[N]-(chlorophenyl)carboxyloxy-pent-2-ynyltrimethylammonium Br)

KW - 4-DAMP

KW - AFDX116

KW - Calcium release

KW - McN-A-343

KW - Muscarinic receptor subtype

KW - N1E-115 neuroblastoma

KW - Pirenzepine

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JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Intracellular calcium release in N1E-115 neuroblastoma cells is mediated by the M1 muscarinic receptor subtype and is antagonized by McN-A-343

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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