Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (±)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (±)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC50 of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Developmental Biology
- Clinical Neurology