Intracellular calcium release in N1E-115 neuroblastoma cells is mediated by the M1 muscarinic receptor subtype and is antagonized by McN-A-343

Chris Mathes, Samuel Sheng-Hung Wang, Hugo M. Vargas, Stuart H. Thompson

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Experiments using muscarinic receptor antagonists were done to determine which muscarinic receptor subtype(s) mediate carbachol-evoked calcium release in N1E-115 cells. McN-A-343 and a new analog, (±)BN228, were weak antagonists and neither compound caused release on its own. The rank order of potency was 4-DAMP > pirenzepine > AFDX116 > (±)BN228 and McN-A-343. This profile, pirenzepine's high potency (19-fold greater than AFDX116) and its IC50 of 31 nM suggest that calcium release in this neuronal cell line is mediated by the M1 muscarinic receptor subtype.

Original languageEnglish (US)
Pages (from-to)307-310
Number of pages4
JournalBrain Research
Volume585
Issue number1-2
DOIs
StatePublished - Jul 10 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

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