Interrogating epigenetic mechanisms with chemically customized chromatin

Nir Hananya, Shany Koren, Tom W. Muir

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

Genetic and genomic techniques have proven incredibly powerful for identifying and studying molecular players implicated in the epigenetic regulation of DNA-templated processes such as transcription. However, achieving a mechanistic understanding of how these molecules interact with chromatin to elicit a functional output is non-trivial, owing to the tremendous complexity of the biochemical networks involved. Advances in protein engineering have enabled the reconstitution of ‘designer’ chromatin containing customized post-translational modification patterns, which, when used in conjunction with sophisticated biochemical and biophysical methods, allow many mechanistic questions to be addressed. In this Review, we discuss how such tools complement established ‘omics’ techniques to answer fundamental questions on chromatin regulation, focusing on chromatin mark establishment and protein–chromatin interactions.

Original languageEnglish (US)
Pages (from-to)255-271
Number of pages17
JournalNature Reviews Genetics
Volume25
Issue number4
DOIs
StatePublished - Apr 2024

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Genetics
  • Molecular Biology

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