TY - JOUR
T1 - Internal tracts of telomeric DNA act as silencers in Saccharomyces cerevisiae
AU - Stavenhagen, Jeffrey B.
AU - Zakian, Virginia A.
PY - 1994/6/15
Y1 - 1994/6/15
N2 - Telomeric position effect (TPE) refers to the ability of telomeres to repress the transcription of genes in their vicinity. Internal stretches of C1-3A DNA, the sequence found at Saccharomyces telomeres, also repressed transcription of nearby genes. This repression, hereafter called C1-3A- based silencing, was observed at several chromosomal loci, including on a circular chromosome. The magnitude of C1-3A-based silencing was increased by both proximity to a telomere and increased length of the C1-3A tracts. C1-3A-based silencing was affected by many of the same genes and conditions that influence TPE and acted in an orientation-independent manner. Thus, in yeast, an expanded array of a simple repetitive DNA, C1-3A, is sufficient to promote the assembly of a transcriptionally silent chromosomal domain.
AB - Telomeric position effect (TPE) refers to the ability of telomeres to repress the transcription of genes in their vicinity. Internal stretches of C1-3A DNA, the sequence found at Saccharomyces telomeres, also repressed transcription of nearby genes. This repression, hereafter called C1-3A- based silencing, was observed at several chromosomal loci, including on a circular chromosome. The magnitude of C1-3A-based silencing was increased by both proximity to a telomere and increased length of the C1-3A tracts. C1-3A-based silencing was affected by many of the same genes and conditions that influence TPE and acted in an orientation-independent manner. Thus, in yeast, an expanded array of a simple repetitive DNA, C1-3A, is sufficient to promote the assembly of a transcriptionally silent chromosomal domain.
KW - Telomeres
KW - position effect
KW - silencers
KW - yeast
UR - http://www.scopus.com/inward/record.url?scp=0028237655&partnerID=8YFLogxK
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U2 - 10.1101/gad.8.12.1411
DO - 10.1101/gad.8.12.1411
M3 - Article
C2 - 7926741
AN - SCOPUS:0028237655
SN - 0890-9369
VL - 8
SP - 1411
EP - 1422
JO - Genes and Development
JF - Genes and Development
IS - 12
ER -