TY - JOUR
T1 - Intercellular signalling in Vibrio harveyi
T2 - sequence and function of genes regulating expression of luminescence
AU - Bassler, Bonnie Lynn
AU - Wright, Miriam
AU - Showalter, Richard E.
AU - Silverman, Michael R.
PY - 1993/8
Y1 - 1993/8
N2 - Density‐dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of an extracellular signal molecule (autoinducer) in the culture medium. A recombinant clone that restored function to one class of spontaneous dim mutants was found to encode functions necessary for the synthesis of, and response to, a signal molecule. Sequence analysis of the region encoding these functions revealed three open reading frames, two (luxL and luxM) that are required for production of an autoinducer substance and a third (luxN) that is required for response to this signal substance. The LuxL and LuxM proteins are not similar in amino acid sequence to other proteins in the database, but the LuxN protein contains regions of sequence resembling both the histidine protein kinase and the response regulator domains of the family of two–component, signal transduction proteins. The phenotypes of mutants with luxL, luxM and luxN defects indicated that an additional signal–response system controlling density‐dependent expression of luminescence remains to be identified.
AB - Density‐dependent expression of luminescence in Vibrio harveyi is regulated by the concentration of an extracellular signal molecule (autoinducer) in the culture medium. A recombinant clone that restored function to one class of spontaneous dim mutants was found to encode functions necessary for the synthesis of, and response to, a signal molecule. Sequence analysis of the region encoding these functions revealed three open reading frames, two (luxL and luxM) that are required for production of an autoinducer substance and a third (luxN) that is required for response to this signal substance. The LuxL and LuxM proteins are not similar in amino acid sequence to other proteins in the database, but the LuxN protein contains regions of sequence resembling both the histidine protein kinase and the response regulator domains of the family of two–component, signal transduction proteins. The phenotypes of mutants with luxL, luxM and luxN defects indicated that an additional signal–response system controlling density‐dependent expression of luminescence remains to be identified.
UR - http://www.scopus.com/inward/record.url?scp=0027247722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027247722&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1993.tb01737.x
DO - 10.1111/j.1365-2958.1993.tb01737.x
M3 - Article
C2 - 8231809
AN - SCOPUS:0027247722
SN - 0950-382X
VL - 9
SP - 773
EP - 786
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 4
ER -