Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

Krystal K. Lum; Bokai Song; Joel D. Federspiel; Benjamin A. Diner; Timothy Howard; Ileana M. Cristea

doi:10.1016/j.cels.2018.10.010

Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

Krystal K. Lum
, Bokai Song
, Joel D. Federspiel
, Benjamin A. Diner
, Timothy Howard
, Ileana M. Cristea

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.

Original language	English (US)
Pages (from-to)	627-642.e6
Journal	Cell Systems
Volume	7
Issue number	6
DOIs	https://doi.org/10.1016/j.cels.2018.10.010
State	Published - Dec 26 2018

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology
Cell Biology

Keywords

DNA sensing
HSV-1
cGAS
herpesvirus
immune signaling
innate immunity
interactome
mass spectrometry
protein interactions
proteomics

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10.1016/j.cels.2018.10.010

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title = "Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS",

abstract = "Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.",

keywords = "DNA sensing, HSV-1, cGAS, herpesvirus, immune signaling, innate immunity, interactome, mass spectrometry, protein interactions, proteomics",

author = "Lum, \{Krystal K.\} and Bokai Song and Federspiel, \{Joel D.\} and Diner, \{Benjamin A.\} and Timothy Howard and Cristea, \{Ileana M.\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2018",

month = dec,

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doi = "10.1016/j.cels.2018.10.010",

language = "English (US)",

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T1 - Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

AU - Lum, Krystal K.

AU - Song, Bokai

AU - Federspiel, Joel D.

AU - Diner, Benjamin A.

AU - Howard, Timothy

AU - Cristea, Ileana M.

PY - 2018/12/26

Y1 - 2018/12/26

N2 - Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.

AB - Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.

KW - DNA sensing

KW - HSV-1

KW - cGAS

KW - herpesvirus

KW - immune signaling

KW - innate immunity

KW - interactome

KW - mass spectrometry

KW - protein interactions

KW - proteomics

UR - https://www.scopus.com/pages/publications/85059246998

UR - https://www.scopus.com/pages/publications/85059246998#tab=citedBy

U2 - 10.1016/j.cels.2018.10.010

DO - 10.1016/j.cels.2018.10.010

M3 - Article

C2 - 30471916

AN - SCOPUS:85059246998

SN - 2405-4712

VL - 7

SP - 627-642.e6

JO - Cell Systems

JF - Cell Systems

IS - 6

ER -

Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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