Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

Krystal K. Lum; Bokai Song; Joel D. Federspiel; Benjamin A. Diner; Timothy Howard; Ileana M. Cristea

doi:10.1016/j.cels.2018.10.010

Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

Krystal K. Lum, Bokai Song, Joel D. Federspiel, Benjamin A. Diner, Timothy Howard, Ileana M. Cristea

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.

Original language	English (US)
Pages (from-to)	627-642.e6
Journal	Cell Systems
Volume	7
Issue number	6
DOIs	https://doi.org/10.1016/j.cels.2018.10.010
State	Published - Dec 26 2018

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology
Cell Biology

Keywords

DNA sensing
HSV-1
cGAS
herpesvirus
immune signaling
innate immunity
interactome
mass spectrometry
protein interactions
proteomics

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10.1016/j.cels.2018.10.010

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title = "Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS",

abstract = "Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.",

keywords = "DNA sensing, HSV-1, cGAS, herpesvirus, immune signaling, innate immunity, interactome, mass spectrometry, protein interactions, proteomics",

author = "Lum, {Krystal K.} and Bokai Song and Federspiel, {Joel D.} and Diner, {Benjamin A.} and Timothy Howard and Cristea, {Ileana M.}",

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AU - Lum, Krystal K.

AU - Song, Bokai

AU - Federspiel, Joel D.

AU - Diner, Benjamin A.

AU - Howard, Timothy

AU - Cristea, Ileana M.

PY - 2018/12/26

Y1 - 2018/12/26

N2 - Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.

AB - Lum et al. characterize the global protein interactome of the DNA sensor, cGAS, in cellular homeostasis and during HSV-1 infection. Integrating quantitative proteomics, CRISPR-Cas9 technology, reciprocal isolations, and cytokine assays, the function of the discovered cGAS-OASL interaction suggests negative interferon regulation and crosstalk between the DNA and RNA sensing pathways.

KW - DNA sensing

KW - HSV-1

KW - cGAS

KW - herpesvirus

KW - immune signaling

KW - innate immunity

KW - interactome

KW - mass spectrometry

KW - protein interactions

KW - proteomics

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JF - Cell Systems

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ER -

Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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