Interactions among the three adenovirus core proteins

P. K. Chatterjee, M. E. Vayda, S. J. Flint

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Interactions among the three adenovirus core polypeptides V, VII, and μ were examined, using the reversible chemical cross-linker dithiobis(succinimidyl propionate) and two-dimensional polyacrylamide gel electrophoresis. Cross-linked species obtained from gradient-purified adenovirus type 2 cores were well represented among the cross-linked products of pentonless virions and crude core preparations. The more efficiently formed cross-linked core species were also identified with the arginine-specific cross-linker, p-azidophenyl glyoxal. In addition to dimers of polypeptides V and VII, efficient cross-linking of V to VII, V to μ, and VII to V to μ was detected in adenovirus cores. Notably absent were cross-linked species corresponding to higher multimers of polypeptide VII. A major core-capsid interaction appeared to be via the association of polypeptide V with a dimer of polypeptide VI.

Original languageEnglish (US)
Pages (from-to)379-386
Number of pages8
JournalJournal of virology
Volume55
Issue number2
DOIs
StatePublished - 1985

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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