Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis

Josef A. Gramespacher, Adam J. Stevens, Duy P. Nguyen, Jason W. Chin, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Naturally split inteins have found widespread use in chemical biology due to their ability to drive the ligation of separately expressed polypeptides through a process termed protein trans-splicing (PTS). In this study, we harness PTS by rendering association of split intein fragments conditional upon the presence of a user-defined protease. We show that these intein "zymogens" can be used to create protein sensors and actuators that respond to the presence of various stimuli, including bacterial pathogens, viral infections, and light. We also show that this design strategy is compatible with several orthogonal split intein pairs, thereby opening the way to the creation of multiplexed sensor systems.

Original languageEnglish (US)
Pages (from-to)8074-8077
Number of pages4
JournalJournal of the American Chemical Society
Volume139
Issue number24
DOIs
StatePublished - Jun 21 2017

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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