Insights into translational termination from the structure of RF2 bound to the ribosome

Albert Weixlbaumer; Hong Jin; Cajetan Neubauer; Rebecca M. Voorhees; Sabine Petry; Ann C. Kelley; Venki Ramakrishnan

doi:10.1126/science.1164840

Insights into translational termination from the structure of RF2 bound to the ribosome

Albert Weixlbaumer, Hong Jin, Cajetan Neubauer, Rebecca M. Voorhees, Sabine Petry, Ann C. Kelley, Venki Ramakrishnan

Research output: Contribution to journal › Article › peer-review

244 Scopus citations

Abstract

The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.

Original language	English (US)
Pages (from-to)	953-956
Number of pages	4
Journal	Science
Volume	322
Issue number	5903
DOIs	https://doi.org/10.1126/science.1164840
State	Published - Nov 7 2008
Externally published	Yes

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abstract = "The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.",

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AU - Weixlbaumer, Albert

AU - Jin, Hong

AU - Neubauer, Cajetan

AU - Voorhees, Rebecca M.

AU - Petry, Sabine

AU - Kelley, Ann C.

AU - Ramakrishnan, Venki

PY - 2008/11/7

Y1 - 2008/11/7

N2 - The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.

AB - The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.

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Insights into translational termination from the structure of RF2 bound to the ribosome

Abstract

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