TY - JOUR
T1 - Innovations present in the primate interneuron repertoire
AU - Krienen, Fenna M.
AU - Goldman, Melissa
AU - Zhang, Qiangge
AU - C. H. del Rosario, Ricardo
AU - Florio, Marta
AU - Machold, Robert
AU - Saunders, Arpiar
AU - Levandowski, Kirsten
AU - Zaniewski, Heather
AU - Schuman, Benjamin
AU - Wu, Carolyn
AU - Lutservitz, Alyssa
AU - Mullally, Christopher D.
AU - Reed, Nora
AU - Bien, Elizabeth
AU - Bortolin, Laura
AU - Fernandez-Otero, Marian
AU - Lin, Jessica D.
AU - Wysoker, Alec
AU - Nemesh, James
AU - Kulp, David
AU - Burns, Monika
AU - Tkachev, Victor
AU - Smith, Richard
AU - Walsh, Christopher A.
AU - Dimidschstein, Jordane
AU - Rudy, Bernardo
AU - S. Kean, Leslie
AU - Berretta, Sabina
AU - Fishell, Gord
AU - Feng, Guoping
AU - McCarroll, Steven A.
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/10/8
Y1 - 2020/10/8
N2 - Primates and rodents, which descended from a common ancestor around 90 million years ago1, exhibit profound differences in behaviour and cognitive capacity; the cellular basis for these differences is unknown. Here we use single-nucleus RNA sequencing to profile RNA expression in 188,776 individual interneurons across homologous brain regions from three primates (human, macaque and marmoset), a rodent (mouse) and a weasel (ferret). Homologous interneuron types—which were readily identified by their RNA-expression patterns—varied in abundance and RNA expression among ferrets, mice and primates, but varied less among primates. Only a modest fraction of the genes identified as ‘markers’ of specific interneuron subtypes in any one species had this property in another species. In the primate neocortex, dozens of genes showed spatial expression gradients among interneurons of the same type, which suggests that regional variation in cortical contexts shapes the RNA expression patterns of adult neocortical interneurons. We found that an interneuron type that was previously associated with the mouse hippocampus—the ‘ivy cell’, which has neurogliaform characteristics—has become abundant across the neocortex of humans, macaques and marmosets but not mice or ferrets. We also found a notable subcortical innovation: an abundant striatal interneuron type in primates that had no molecularly homologous counterpart in mice or ferrets. These interneurons expressed a unique combination of genes that encode transcription factors, receptors and neuropeptides and constituted around 30% of striatal interneurons in marmosets and humans.
AB - Primates and rodents, which descended from a common ancestor around 90 million years ago1, exhibit profound differences in behaviour and cognitive capacity; the cellular basis for these differences is unknown. Here we use single-nucleus RNA sequencing to profile RNA expression in 188,776 individual interneurons across homologous brain regions from three primates (human, macaque and marmoset), a rodent (mouse) and a weasel (ferret). Homologous interneuron types—which were readily identified by their RNA-expression patterns—varied in abundance and RNA expression among ferrets, mice and primates, but varied less among primates. Only a modest fraction of the genes identified as ‘markers’ of specific interneuron subtypes in any one species had this property in another species. In the primate neocortex, dozens of genes showed spatial expression gradients among interneurons of the same type, which suggests that regional variation in cortical contexts shapes the RNA expression patterns of adult neocortical interneurons. We found that an interneuron type that was previously associated with the mouse hippocampus—the ‘ivy cell’, which has neurogliaform characteristics—has become abundant across the neocortex of humans, macaques and marmosets but not mice or ferrets. We also found a notable subcortical innovation: an abundant striatal interneuron type in primates that had no molecularly homologous counterpart in mice or ferrets. These interneurons expressed a unique combination of genes that encode transcription factors, receptors and neuropeptides and constituted around 30% of striatal interneurons in marmosets and humans.
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U2 - 10.1038/s41586-020-2781-z
DO - 10.1038/s41586-020-2781-z
M3 - Article
C2 - 32999462
AN - SCOPUS:85091726335
SN - 0028-0836
VL - 586
SP - 262
EP - 269
JO - Nature
JF - Nature
IS - 7828
ER -