Initiation and propagation of calcium-dependent action potentials in a coupled network of olfactory interneurons

Jing W. Wang, Winfried Denk, Jorge Flores, Alan Gelperin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Coherent oscillatory electrical activity and apicalbasal wave propagation have been described previously in the procerebral (PC) lobe, an olfactory center of the terrestrial slug Limax maximus. In this study, we investigate the physiological basis of oscillatory activity and wave propagation in the PC lobe. Calcium green dextran was locally deposited in the PC lobe; this led to cellular uptake and transport of dye by bursting and nonbursting neurons of the PC lobe. The change of intracellular calcium concentration was measured at several different positions in neurites of individual bursting neurons in the PC lobe with a two-photon laser-scanning microscope. Fluorescence measurements were also made from neurons intracellularly injected with calcium green-1. Two different morphological classes of bursting neurons were found, varicose (VB) and smooth (SB). Our results from concurrent optical and intracellular recordings suggest that Ca2+ is the major carrier for the inward current during action potentials of bursting neurons. Intracellular recordings from bursting neurons with nystatin perforated-patch electrodes made while simultaneously recording the local field potential (LFP) with extracellular electrodes indicate that the burster spikes are precisely phase-locked to the periodic LFP events. By referencing successive calcium measurements to the common LFP signal, we could therefore accurately determine the relative timing of calcium transients at different points along a neurite. Measuring the relation of temporal to spatial differences allowed us to estimate the velocity of action potential propagation, which was 4.3 ± 0.2 (SE) mm/s in VBs, and 1.3 ± 0.2 mm/s in SB.

Original languageEnglish (US)
Pages (from-to)977-985
Number of pages9
JournalJournal of neurophysiology
Volume85
Issue number2
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

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