Inhibitor Mimetic Mutations in the Pseudomonas aeruginosa PqsE Enzyme Reveal a Protein-Protein Interaction with the Quorum-Sensing Receptor RhlR That Is Vital for Virulence Factor Production

Isabelle R. Taylor, Jon E. Paczkowski, Philip D. Jeffrey, Brad R. Henke, Chari D. Smith, Bonnie L. Bassler

Research output: Contribution to journalArticlepeer-review

Abstract

Pseudomonas aeruginosa is an opportunistic human pathogen that causes fatal infections. There exists an urgent need for new antimicrobial agents to combat P. aeruginosa. We conducted a screen for molecules that bind the virulence-controlling protein PqsE and characterized hit compounds for inhibition of PqsE enzymatic activity. The binding conformations of two inhibitory molecules, BB391 and BB393, were identified by crystallography, and inhibitor binding was mimicked by the substitution of PqsE residues E182 and S285 with tryptophan. Comparison of the inhibitor-mimetic mutations to the catalytically inactive PqsE D73A protein demonstrated that catalysis is not responsible for the role PqsE plays in driving virulence factor production. Rather, the PqsE E182W protein fails to interact with the quorum-sensing receptor, RhlR, and our results suggest that it is this interaction that is responsible for promoting virulence factor production in P. aeruginosa. These findings provide a new route for drug discovery efforts targeting PqsE.

Original languageEnglish (US)
Pages (from-to)740-752
Number of pages13
JournalACS chemical biology
Volume16
Issue number4
DOIs
StatePublished - Apr 16 2021

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine

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