Inhibition of Nitrosomonas europaea by monoterpenes from coastal redwood (Sequoia sempervirens) in whole-cell studies

B. B. Ward, K. J. Courtney, J. H. Langenheim

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Inhibition by allelochemicals, including monoterpenes, has been suggested as a factor in the extremely low nitrification rates observed in coastal redwood forests. Similarities between the molecular structure of known nitrification inhibitors and some conifer monoterpenes have been suggested as one reason for the inhibition of autotrophic nitrifiers by conifer monoterpenes. The effect of monoterpenes on nitrification rate and growth of Nitrosomonas europaea was examined in whole-cell pure culture experiments using the five most abundant monoterpenes in coastal redwood needles. These are (in order of decreasing concentration in the needles limonene, α-pinene, sabinene, myrcene, and γ-terpinene. Four of the five compounds significantly inhibited growth of N. europaea in batch culture experiments. Short-term kinetic studies of the two most inhibitory monoterpenes, limonene and α-pinene, were performed on whole cells to evaluate the mode of interaction between these chemicals and nitrification rates. Inhibition constants (K(i)) of limonene (38 μM) and α-pinene (95 μM) were determined. Lineweaver-Burk plots of nitrification in the presence of monoterpenes appear to fit a noncompetitive inhibition model; however, the mechanisms of inhibition may be more complex.

Original languageEnglish (US)
Pages (from-to)2583-2598
Number of pages16
JournalJournal of Chemical Ecology
Volume23
Issue number11
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Keywords

  • Conifers
  • Inhibition
  • Kinetics
  • Monoterpenes
  • Nitrification
  • Nitrogen cycling
  • Nitrosomonas europaea
  • Sequoia sempervirens

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