TY - JOUR
T1 - Inhibition of HeLa cell protein synthesis during adenovirus infection. Restriction of cellular messenger RNA sequences to the nucleus
AU - Beltz, Gerald A.
AU - Flint, S. J.
N1 - Funding Information:
We thank H. Weintraub for a gift of avian myeloblastosis virus re\‘erse transcriptasr and 8. Weisbrod for advice on its use. This work was supported by a grant from thti National Science Foundation (no. PCM78-07724) and one of the authors (G. B.) acknow-Ietlgrs sllpport from a Public Health Service Training Grant (Ilo. CA-09167).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979/6/25
Y1 - 1979/6/25
N2 - The mechanism by which adenovirus type 2 inhibits HeLa cell protein synthesis has been investigated by a quantitative analysis of the synthesis and metabolism of cellular and viral hnRNA† † Abbreviations used: hnRNA, heterogeneous nuclear RNA; cDNA, complementary DNA. sequences. By the time labeling of cellular proteins is maximally inhibited, 16 hours following adenovirus infection under the conditions we have used, all of the newly made messenger RNA sequences reaching the cytoplasm are viral-specific. Thus, the gradual shift from translation of cellular to translation of viral mRNA species that begins following entry of adenovirus-infected cells into the late phase of the productive cycle, can probably be explained simply by competition between the two types of mRNA for available ribosomes. In order to investigate the mechanism(s) by which adenovirus infection exerts this dramatic change in the biogenesis of HeLa cellular mRNA sequences, the hnRNA fraction has been isolated quantitatively from infected cells after pulselabeling or a pulse-chase. Cellular and viral transcripts were then distinguished by hybridization to viral DNA. The results of these experiments reveal that the rate of synthesis of cellular hnRNA sequences remains constant throughout the course of adenovirus type 2 infection. Moreover, poly(A) is apparently added normally to cellular hnRNA sequences synthesized during the late phase of productive adenovirus infection. This newly synthesized, poly(A)-containing hnRNA cannot be distinguished from the corresponding fraction isolated from mockinfected cells by hybridization to cDNA prepared using uninfected HeLa cell, cytoplasmic, poly(A)-containing RNA as template: it also appears to be metabolized with kinetics similar to those observed in uninfected cells.
AB - The mechanism by which adenovirus type 2 inhibits HeLa cell protein synthesis has been investigated by a quantitative analysis of the synthesis and metabolism of cellular and viral hnRNA† † Abbreviations used: hnRNA, heterogeneous nuclear RNA; cDNA, complementary DNA. sequences. By the time labeling of cellular proteins is maximally inhibited, 16 hours following adenovirus infection under the conditions we have used, all of the newly made messenger RNA sequences reaching the cytoplasm are viral-specific. Thus, the gradual shift from translation of cellular to translation of viral mRNA species that begins following entry of adenovirus-infected cells into the late phase of the productive cycle, can probably be explained simply by competition between the two types of mRNA for available ribosomes. In order to investigate the mechanism(s) by which adenovirus infection exerts this dramatic change in the biogenesis of HeLa cellular mRNA sequences, the hnRNA fraction has been isolated quantitatively from infected cells after pulselabeling or a pulse-chase. Cellular and viral transcripts were then distinguished by hybridization to viral DNA. The results of these experiments reveal that the rate of synthesis of cellular hnRNA sequences remains constant throughout the course of adenovirus type 2 infection. Moreover, poly(A) is apparently added normally to cellular hnRNA sequences synthesized during the late phase of productive adenovirus infection. This newly synthesized, poly(A)-containing hnRNA cannot be distinguished from the corresponding fraction isolated from mockinfected cells by hybridization to cDNA prepared using uninfected HeLa cell, cytoplasmic, poly(A)-containing RNA as template: it also appears to be metabolized with kinetics similar to those observed in uninfected cells.
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U2 - 10.1016/0022-2836(79)90081-0
DO - 10.1016/0022-2836(79)90081-0
M3 - Article
C2 - 226719
AN - SCOPUS:0018758069
SN - 0022-2836
VL - 131
SP - 353
EP - 373
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -