Individual dorsal morphogen binding sites mediate activation repression in the Drosophila embryo

Jin Jiang, Christine A. Rushlow, Qin Zhou, Stephen Small, Michael Levine

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

The dorsal (dl) morphogen gradient is responsible for initiating the differentiation of the mesoderm, neuroectoderm and dorsal ectoderm in the Drosophila embryo. dl encodes a sequence-specific DNA binding protein that belongs to the Rel family of transcription factors. Previous studies have shown that dl activates the mesoderm determinant twist (twi); here we use a combination of site-directed mutagenesis and P-transformation assays to demonstrate that it also functions as a direct transcriptional represser of a second target gene, zerknüllt (zen). By exchanging dl binding sites between the promoters we show that activator sites from twi can mediate repression when placed in the context of the zen promoter, and that represser sites from zen can mediate activation in the context of the twi promoter. This represents the first demonstration that common binding sites for any DNA binding protein can mediate both activation and repression in a developing embryo. Evidence is also presented that the affinities of dl binding sites are important for the efficiency of repression, but are not the sole determinants of the threshold response to the dl gradient.

Original languageEnglish (US)
Pages (from-to)3147-3154
Number of pages8
JournalEMBO Journal
Volume11
Issue number8
StatePublished - 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • Molecular Biology
  • General Neuroscience

Keywords

  • Dorsal
  • Drosophila
  • Transcription factors
  • Twist
  • Zerknüllt

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