Improved reference genome of Aedes aegypti informs arbovirus vector control

Benjamin J. Matthews; Olga Dudchenko; Sarah B. Kingan; Sergey Koren; Igor Antoshechkin; Jacob E. Crawford; William J. Glassford; Margaret Herre; Seth N. Redmond; Noah H. Rose; Gareth D. Weedall; Yang Wu; Sanjit S. Batra; Carlos A. Brito-Sierra; Steven D. Buckingham; Corey L. Campbell; Saki Chan; Eric Cox; Benjamin R. Evans; Thanyalak Fansiri; Igor Filipović; Albin Fontaine; Andrea Gloria-Soria; Richard Hall; Vinita S. Joardar; Andrew K. Jones; Raissa G.G. Kay; Vamsi K. Kodali; Joyce Lee; Gareth J. Lycett; Sara N. Mitchell; Jill Muehling; Michael R. Murphy; Arina D. Omer; Frederick A. Partridge; Paul Peluso; Aviva Presser Aiden; Vidya Ramasamy; Gordana Rašić; Sourav Roy; Karla Saavedra-Rodriguez; Shruti Sharan; Atashi Sharma; Melissa Laird Smith; Joe Turner; Allison M. Weakley; Zhilei Zhao; Omar S. Akbari; William C. Black; Han Cao; Alistair C. Darby; Catherine A. Hill; J. Spencer Johnston; Terence D. Murphy; Alexander S. Raikhel; David B. Sattelle; Igor V. Sharakhov; Bradley J. White; Li Zhao; Erez Lieberman Aiden; Richard S. Mann; Louis Lambrechts; Jeffrey R. Powell; Maria V. Sharakhova; Zhijian Tu; Hugh M. Robertson; Carolyn S. McBride; Alex R. Hastie; Jonas Korlach; Daniel E. Neafsey; Adam M. Phillippy; Leslie B. Vosshall

doi:10.1038/s41586-018-0692-z

Improved reference genome of Aedes aegypti informs arbovirus vector control

Benjamin J. Matthews, Olga Dudchenko, Sarah B. Kingan, Sergey Koren, Igor Antoshechkin, Jacob E. Crawford, William J. Glassford, Margaret Herre, Seth N. Redmond, Noah H. Rose, Gareth D. Weedall, Yang Wu, Sanjit S. Batra, Carlos A. Brito-Sierra, Steven D. Buckingham, Corey L. Campbell, Saki Chan, Eric Cox, Benjamin R. Evans, Thanyalak FansiriIgor Filipović, Albin Fontaine, Andrea Gloria-Soria, Richard Hall, Vinita S. Joardar, Andrew K. Jones, Raissa G.G. Kay, Vamsi K. Kodali, Joyce Lee, Gareth J. Lycett, Sara N. Mitchell, Jill Muehling, Michael R. Murphy, Arina D. Omer, Frederick A. Partridge, Paul Peluso, Aviva Presser Aiden, Vidya Ramasamy, Gordana Rašić, Sourav Roy, Karla Saavedra-Rodriguez, Shruti Sharan, Atashi Sharma, Melissa Laird Smith, Joe Turner, Allison M. Weakley, Zhilei Zhao, Omar S. Akbari, William C. Black, Han Cao, Alistair C. Darby, Catherine A. Hill, J. Spencer Johnston, Terence D. Murphy, Alexander S. Raikhel, David B. Sattelle, Igor V. Sharakhov, Bradley J. White, Li Zhao, Erez Lieberman Aiden, Richard S. Mann, Louis Lambrechts, Jeffrey R. Powell, Maria V. Sharakhova, Zhijian Tu, Hugh M. Robertson, Carolyn S. McBride, Alex R. Hastie, Jonas Korlach, Daniel E. Neafsey, Adam M. Phillippy, Leslie B. Vosshall

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Abstract

Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.

Original language	English (US)
Pages (from-to)	501-507
Number of pages	7
Journal	Nature
Volume	563
Issue number	7732
DOIs	https://doi.org/10.1038/s41586-018-0692-z
State	Published - Nov 22 2018

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Matthews, B. J., Dudchenko, O., Kingan, S. B., Koren, S., Antoshechkin, I., Crawford, J. E., Glassford, W. J., Herre, M., Redmond, S. N., Rose, N. H., Weedall, G. D., Wu, Y., Batra, S. S., Brito-Sierra, C. A., Buckingham, S. D., Campbell, C. L., Chan, S., Cox, E., Evans, B. R., ... Vosshall, L. B. (2018). Improved reference genome of Aedes aegypti informs arbovirus vector control. Nature, 563(7732), 501-507. https://doi.org/10.1038/s41586-018-0692-z

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title = "Improved reference genome of Aedes aegypti informs arbovirus vector control",

abstract = "Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.",

author = "Matthews, {Benjamin J.} and Olga Dudchenko and Kingan, {Sarah B.} and Sergey Koren and Igor Antoshechkin and Crawford, {Jacob E.} and Glassford, {William J.} and Margaret Herre and Redmond, {Seth N.} and Rose, {Noah H.} and Weedall, {Gareth D.} and Yang Wu and Batra, {Sanjit S.} and Brito-Sierra, {Carlos A.} and Buckingham, {Steven D.} and Campbell, {Corey L.} and Saki Chan and Eric Cox and Evans, {Benjamin R.} and Thanyalak Fansiri and Igor Filipovi{\'c} and Albin Fontaine and Andrea Gloria-Soria and Richard Hall and Joardar, {Vinita S.} and Jones, {Andrew K.} and Kay, {Raissa G.G.} and Kodali, {Vamsi K.} and Joyce Lee and Lycett, {Gareth J.} and Mitchell, {Sara N.} and Jill Muehling and Murphy, {Michael R.} and Omer, {Arina D.} and Partridge, {Frederick A.} and Paul Peluso and Aiden, {Aviva Presser} and Vidya Ramasamy and Gordana Ra{\v s}i{\'c} and Sourav Roy and Karla Saavedra-Rodriguez and Shruti Sharan and Atashi Sharma and Smith, {Melissa Laird} and Joe Turner and Weakley, {Allison M.} and Zhilei Zhao and Akbari, {Omar S.} and Black, {William C.} and Han Cao and Darby, {Alistair C.} and Hill, {Catherine A.} and Johnston, {J. Spencer} and Murphy, {Terence D.} and Raikhel, {Alexander S.} and Sattelle, {David B.} and Sharakhov, {Igor V.} and White, {Bradley J.} and Li Zhao and Aiden, {Erez Lieberman} and Mann, {Richard S.} and Louis Lambrechts and Powell, {Jeffrey R.} and Sharakhova, {Maria V.} and Zhijian Tu and Robertson, {Hugh M.} and McBride, {Carolyn S.} and Hastie, {Alex R.} and Jonas Korlach and Neafsey, {Daniel E.} and Phillippy, {Adam M.} and Vosshall, {Leslie B.}",

note = "Publisher Copyright: {\textcopyright} 2018, Springer Nature Limited.",

year = "2018",

month = nov,

day = "22",

doi = "10.1038/s41586-018-0692-z",

language = "English (US)",

volume = "563",

pages = "501--507",

journal = "Nature",

issn = "0028-0836",

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Matthews, BJ, Dudchenko, O, Kingan, SB, Koren, S, Antoshechkin, I, Crawford, JE, Glassford, WJ, Herre, M, Redmond, SN, Rose, NH, Weedall, GD, Wu, Y, Batra, SS, Brito-Sierra, CA, Buckingham, SD, Campbell, CL, Chan, S, Cox, E, Evans, BR, Fansiri, T, Filipović, I, Fontaine, A, Gloria-Soria, A, Hall, R, Joardar, VS, Jones, AK, Kay, RGG, Kodali, VK, Lee, J, Lycett, GJ, Mitchell, SN, Muehling, J, Murphy, MR, Omer, AD, Partridge, FA, Peluso, P, Aiden, AP, Ramasamy, V, Rašić, G, Roy, S, Saavedra-Rodriguez, K, Sharan, S, Sharma, A, Smith, ML, Turner, J, Weakley, AM, Zhao, Z, Akbari, OS, Black, WC, Cao, H, Darby, AC, Hill, CA, Johnston, JS, Murphy, TD, Raikhel, AS, Sattelle, DB, Sharakhov, IV, White, BJ, Zhao, L, Aiden, EL, Mann, RS, Lambrechts, L, Powell, JR, Sharakhova, MV, Tu, Z, Robertson, HM, McBride, CS, Hastie, AR, Korlach, J, Neafsey, DE, Phillippy, AM & Vosshall, LB 2018, 'Improved reference genome of Aedes aegypti informs arbovirus vector control', Nature, vol. 563, no. 7732, pp. 501-507. https://doi.org/10.1038/s41586-018-0692-z

TY - JOUR

T1 - Improved reference genome of Aedes aegypti informs arbovirus vector control

AU - Matthews, Benjamin J.

AU - Dudchenko, Olga

AU - Kingan, Sarah B.

AU - Koren, Sergey

AU - Antoshechkin, Igor

AU - Crawford, Jacob E.

AU - Glassford, William J.

AU - Herre, Margaret

AU - Redmond, Seth N.

AU - Rose, Noah H.

AU - Weedall, Gareth D.

AU - Wu, Yang

AU - Batra, Sanjit S.

AU - Brito-Sierra, Carlos A.

AU - Buckingham, Steven D.

AU - Campbell, Corey L.

AU - Chan, Saki

AU - Cox, Eric

AU - Evans, Benjamin R.

AU - Fansiri, Thanyalak

AU - Filipović, Igor

AU - Fontaine, Albin

AU - Gloria-Soria, Andrea

AU - Hall, Richard

AU - Joardar, Vinita S.

AU - Jones, Andrew K.

AU - Kay, Raissa G.G.

AU - Kodali, Vamsi K.

AU - Lee, Joyce

AU - Lycett, Gareth J.

AU - Mitchell, Sara N.

AU - Muehling, Jill

AU - Murphy, Michael R.

AU - Omer, Arina D.

AU - Partridge, Frederick A.

AU - Peluso, Paul

AU - Aiden, Aviva Presser

AU - Ramasamy, Vidya

AU - Rašić, Gordana

AU - Roy, Sourav

AU - Saavedra-Rodriguez, Karla

AU - Sharan, Shruti

AU - Sharma, Atashi

AU - Smith, Melissa Laird

AU - Turner, Joe

AU - Weakley, Allison M.

AU - Zhao, Zhilei

AU - Akbari, Omar S.

AU - Black, William C.

AU - Cao, Han

AU - Darby, Alistair C.

AU - Hill, Catherine A.

AU - Johnston, J. Spencer

AU - Murphy, Terence D.

AU - Raikhel, Alexander S.

AU - Sattelle, David B.

AU - Sharakhov, Igor V.

AU - White, Bradley J.

AU - Zhao, Li

AU - Aiden, Erez Lieberman

AU - Mann, Richard S.

AU - Lambrechts, Louis

AU - Powell, Jeffrey R.

AU - Sharakhova, Maria V.

AU - Tu, Zhijian

AU - Robertson, Hugh M.

AU - McBride, Carolyn S.

AU - Hastie, Alex R.

AU - Korlach, Jonas

AU - Neafsey, Daniel E.

AU - Phillippy, Adam M.

AU - Vosshall, Leslie B.

PY - 2018/11/22

Y1 - 2018/11/22

N2 - Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.

AB - Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.

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U2 - 10.1038/s41586-018-0692-z

DO - 10.1038/s41586-018-0692-z

M3 - Article

C2 - 30429615

AN - SCOPUS:85056867976

SN - 0028-0836

VL - 563

SP - 501

EP - 507

JO - Nature

JF - Nature

IS - 7732

ER -

Improved reference genome of Aedes aegypti informs arbovirus vector control

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