Improved derivative of a phage λ EK2 vector for cloning recombinant DNA

David Tiemeier, Lynn Enquist, Philip Leder

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

THOMAS et al.1 have constructed a derivative of phage λ (λgt·λC) that is useful for cloning DNA fragments 1,000 to 15,000 base pairs long from other organisms. Their cloning procedure involves cutting the phage DNA into three fragments with EcoRI nuclease, removing the central (or "C") fragment, and annealing the outside fragments with an RI digest of "n DNA. We have recently introduced three amber mutations - Wam403, Eam1100, and Sam100 - into λgt·λC with the object of making the phage less likely to encounter a susceptible host in nature 2. The NIH Advisory Committee on Recombinant DNA Research, on the basis of appropriate tests, has certified λgtWES·λC as an EK2 vector3,4.

Original languageEnglish (US)
Pages (from-to)526-527
Number of pages2
JournalNature
Volume263
Issue number5577
DOIs
StatePublished - Dec 1 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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