Improved characterisation and quantification of proteins using differential isotope labeling

Shabaz Mohammed; Ileana M. Cristea; Francesco L. Brancia; Stephen G. Oliver; Simon J. Gaskell

Improved characterisation and quantification of proteins using differential isotope labeling

Shabaz Mohammed, Ileana M. Cristea, Francesco L. Brancia, Stephen G. Oliver, Simon J. Gaskell

Research output: Contribution to conference › Paper › peer-review

Abstract

The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional benefits in protein identification through improved MALDI MS spectra and easing interpretation of tandem mass spectra.

Original language	English (US)
Pages	391-392
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

All Science Journal Classification (ASJC) codes

Spectroscopy

Cite this

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title = "Improved characterisation and quantification of proteins using differential isotope labeling",

abstract = "The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional benefits in protein identification through improved MALDI MS spectra and easing interpretation of tandem mass spectra.",

author = "Shabaz Mohammed and Cristea, {Ileana M.} and Brancia, {Francesco L.} and Oliver, {Stephen G.} and Gaskell, {Simon J.}",

year = "2002",

language = "English (US)",

pages = "391--392",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Improved characterisation and quantification of proteins using differential isotope labeling

AU - Mohammed, Shabaz

AU - Cristea, Ileana M.

AU - Brancia, Francesco L.

AU - Oliver, Stephen G.

AU - Gaskell, Simon J.

PY - 2002

Y1 - 2002

N2 - The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional benefits in protein identification through improved MALDI MS spectra and easing interpretation of tandem mass spectra.

AB - The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional benefits in protein identification through improved MALDI MS spectra and easing interpretation of tandem mass spectra.

UR - http://www.scopus.com/inward/record.url?scp=2442692012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442692012&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442692012

SP - 391

EP - 392

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Improved characterisation and quantification of proteins using differential isotope labeling

Abstract

Other

All Science Journal Classification (ASJC) codes

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