Improved characterisation and quantification of proteins using differential isotope labeling

Shabaz Mohammed, Ileana M. Cristea, Francesco L. Brancia, Stephen G. Oliver, Simon J. Gaskell

Research output: Contribution to conferencePaper

2 Scopus citations

Abstract

The characterization and quantification of proteins in MALDI spectra were improved using differential isotope labeling. Peptide mass fingerprints were acquired on a Voyager DE-STR reflector MALDI-ToF mass spectrometer. All product ion spectra were acquired on a Q-ToF I instrument equipped with a dual electrospray source. The collision energy was optimized for each peptide analyzed. The results show that guanidination chemistry allows relative quantification to be carried out with additional benefits in protein identification through improved MALDI MS spectra and easing interpretation of tandem mass spectra.

Original languageEnglish (US)
Pages391-392
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

All Science Journal Classification (ASJC) codes

  • Spectroscopy

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  • Cite this

    Mohammed, S., Cristea, I. M., Brancia, F. L., Oliver, S. G., & Gaskell, S. J. (2002). Improved characterisation and quantification of proteins using differential isotope labeling. 391-392. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.