Imaging TGFβsignaling in mouse models of cancer metastasis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metastatic spread of cancer cells from the primary tumors to distant vital organs, such as lung, liver, brain, and bone, is responsible for the majority of cancer-related deaths. Development of metastatic lesions is critically dependent on the interaction of tumor cells with the stromal microenvironment. As a multifunctional paracrine signaling factor that is abundantly produced by both tumor and stromal cells, TGFβ has been well established as an important mediator of tumor-stromal interaction during cancer metastasis. Imaging the in vivo dynamic of TGFβ signaling activity during cancer metastasis is critical for understanding the pathogenesis of the disease, and for the development of effective anti-metastasis treatments. In this chapter, I describe several xenograft methods to introduce human breast cancer cells into nude mice in order to generate spontaneous and experimental metastases, as well as the luciferase-based bioluminescence imaging method for quantitative imaging analysis of TGFβ signaling in tumor cells during metastasis.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages219-232
Number of pages14
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1344
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Animal model
  • Bioluminescence
  • In vivo imaging
  • Intracardiac injection
  • Intravenous injection
  • Luciferase
  • Mammary fat pad injection
  • Metastasis
  • TGFβ
  • Tumor stroma
  • Xenograft

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  • Cite this

    Kang, Y. (2016). Imaging TGFβsignaling in mouse models of cancer metastasis. In Methods in Molecular Biology (pp. 219-232). (Methods in Molecular Biology; Vol. 1344). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2966-5_13