Imaging Neuronal Activity in Cerebellar Cortex of Behaving Mice

Mikhail Kislin; Gerard Joey Broussard; Ben Deverett; Samuel S.H. Wang

doi:10.1007/978-1-0716-2026-7_13

Imaging Neuronal Activity in Cerebellar Cortex of Behaving Mice

Mikhail Kislin, Gerard Joey Broussard, Ben Deverett, Samuel S.H. Wang

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In vivo imaging allows recording of population activity from many cerebellar circuit elements at once. In particular, Purkinje cells, molecular layer interneurons, and granule cells are differentially targetable for imaging studies. In this chapter, we first describe robust gene delivery strategies for these specific cerebellar cell types. We describe protocols for in vivo imaging and constraints that arise in head-fixed and freely moving behavioral paradigms. Finally, we offer practical guidelines for experimental design and data analysis, including the separation of signals from nearby cells.

Original language	English (US)
Title of host publication	Neuromethods
Publisher	Humana Press Inc.
Pages	245-269
Number of pages	25
DOIs	https://doi.org/10.1007/978-1-0716-2026-7_13
State	Published - 2022

Publication series

Name	Neuromethods
Volume	177
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)
Psychiatry and Mental health

Keywords

Calcium imaging
Miniscope
Purkinje cells
Two-photon microscopy
Virus

Access to Document

10.1007/978-1-0716-2026-7_13

Cite this

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abstract = "In vivo imaging allows recording of population activity from many cerebellar circuit elements at once. In particular, Purkinje cells, molecular layer interneurons, and granule cells are differentially targetable for imaging studies. In this chapter, we first describe robust gene delivery strategies for these specific cerebellar cell types. We describe protocols for in vivo imaging and constraints that arise in head-fixed and freely moving behavioral paradigms. Finally, we offer practical guidelines for experimental design and data analysis, including the separation of signals from nearby cells.",

keywords = "Calcium imaging, Miniscope, Purkinje cells, Two-photon microscopy, Virus",

author = "Mikhail Kislin and Broussard, {Gerard Joey} and Ben Deverett and Wang, {Samuel S.H.}",

note = "Funding Information: We gratefully acknowledge the financial supports from the National Natural Science Foundation of China (Nos. 21808240, 21776308 and 21908245), and Science Foundation of China University of Petroleum, Beijing (No. 2462018YJRC009). Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",

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doi = "10.1007/978-1-0716-2026-7_13",

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AU - Kislin, Mikhail

AU - Broussard, Gerard Joey

AU - Deverett, Ben

AU - Wang, Samuel S.H.

N1 - Funding Information: We gratefully acknowledge the financial supports from the National Natural Science Foundation of China (Nos. 21808240, 21776308 and 21908245), and Science Foundation of China University of Petroleum, Beijing (No. 2462018YJRC009). Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - In vivo imaging allows recording of population activity from many cerebellar circuit elements at once. In particular, Purkinje cells, molecular layer interneurons, and granule cells are differentially targetable for imaging studies. In this chapter, we first describe robust gene delivery strategies for these specific cerebellar cell types. We describe protocols for in vivo imaging and constraints that arise in head-fixed and freely moving behavioral paradigms. Finally, we offer practical guidelines for experimental design and data analysis, including the separation of signals from nearby cells.

AB - In vivo imaging allows recording of population activity from many cerebellar circuit elements at once. In particular, Purkinje cells, molecular layer interneurons, and granule cells are differentially targetable for imaging studies. In this chapter, we first describe robust gene delivery strategies for these specific cerebellar cell types. We describe protocols for in vivo imaging and constraints that arise in head-fixed and freely moving behavioral paradigms. Finally, we offer practical guidelines for experimental design and data analysis, including the separation of signals from nearby cells.

KW - Calcium imaging

KW - Miniscope

KW - Purkinje cells

KW - Two-photon microscopy

KW - Virus

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ER -

Imaging Neuronal Activity in Cerebellar Cortex of Behaving Mice

Abstract

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All Science Journal Classification (ASJC) codes

Keywords

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