Imaging Neuronal Activity in Cerebellar Cortex of Behaving Mice

Mikhail Kislin, Gerard Joey Broussard, Ben Deverett, Samuel S.H. Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In vivo imaging allows recording of population activity from many cerebellar circuit elements at once. In particular, Purkinje cells, molecular layer interneurons, and granule cells are differentially targetable for imaging studies. In this chapter, we first describe robust gene delivery strategies for these specific cerebellar cell types. We describe protocols for in vivo imaging and constraints that arise in head-fixed and freely moving behavioral paradigms. Finally, we offer practical guidelines for experimental design and data analysis, including the separation of signals from nearby cells.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages245-269
Number of pages25
DOIs
StatePublished - 2022

Publication series

NameNeuromethods
Volume177
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Keywords

  • Calcium imaging
  • Miniscope
  • Purkinje cells
  • Two-photon microscopy
  • Virus

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