@inbook{49f0306d7cbc4da993a753d0c8c6a29f,
title = "Imaging Neuronal Activity in Cerebellar Cortex of Behaving Mice",
abstract = "In vivo imaging allows recording of population activity from many cerebellar circuit elements at once. In particular, Purkinje cells, molecular layer interneurons, and granule cells are differentially targetable for imaging studies. In this chapter, we first describe robust gene delivery strategies for these specific cerebellar cell types. We describe protocols for in vivo imaging and constraints that arise in head-fixed and freely moving behavioral paradigms. Finally, we offer practical guidelines for experimental design and data analysis, including the separation of signals from nearby cells.",
keywords = "Calcium imaging, Miniscope, Purkinje cells, Two-photon microscopy, Virus",
author = "Mikhail Kislin and Broussard, {Gerard Joey} and Ben Deverett and Wang, {Samuel S.H.}",
note = "Funding Information: We thank Andrea Giovannucci and Pengcheng Zhou for discussion and advice on image analysis and Laura Lynch and Zahra Dhaner-awala for technical assistance. This work was supported by grants to S.S.-H.W. from the National Institutes of Health (grant nos. NIH R01 NS045193, R01 MH115750, and U19 NS104648), and a fellowship to G.J.B. (grant no. F32 MH120887). Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2026-7_13",
language = "English (US)",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "245--269",
booktitle = "Neuromethods",
}