Imaging Cilia in Zebrafish

Kimberly M. Jaffe, Stephan Y. Thiberge, Margaret E. Bisher, Rebecca D. Burdine

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Research focused on cilia as extremely important cellular organelles has flourished in recent years. A thorough understanding of cilia regulation and function is critical, as disruptions of cilia structure and/or function have been linked to numerous human diseases and disorders. The tropical freshwater zebrafish is an excellent model organism in which to study cilia structure and function. We can readily image cilia and their motility in embryonic structures including Kupffer's vesicle during somite stages and the pronephros from 1 day postfertilization onward. Here, we describe how to image cilia by whole-mount immunofluorescence, transverse cryosection/immunohistochemistry, and transmission electron microscopy. We also describe how to obtain videos of cilia motility in living embryos.

Original languageEnglish (US)
Pages (from-to)415-435
Number of pages21
JournalMethods in Cell Biology
Volume97
Issue numberC
DOIs
StatePublished - Aug 20 2010

All Science Journal Classification (ASJC) codes

  • Cell Biology

Fingerprint Dive into the research topics of 'Imaging Cilia in Zebrafish'. Together they form a unique fingerprint.

Cite this