@article{93560ec1f3a34a2d8ba3074b823f758b,
title = "Imaging Cilia in Zebrafish",
abstract = "Research focused on cilia as extremely important cellular organelles has flourished in recent years. A thorough understanding of cilia regulation and function is critical, as disruptions of cilia structure and/or function have been linked to numerous human diseases and disorders. The tropical freshwater zebrafish is an excellent model organism in which to study cilia structure and function. We can readily image cilia and their motility in embryonic structures including Kupffer's vesicle during somite stages and the pronephros from 1 day postfertilization onward. Here, we describe how to image cilia by whole-mount immunofluorescence, transverse cryosection/immunohistochemistry, and transmission electron microscopy. We also describe how to obtain videos of cilia motility in living embryos.",
author = "Jaffe, {Kimberly M.} and Thiberge, {Stephan Y.} and Bisher, {Margaret E.} and Burdine, {Rebecca D.}",
note = "Funding Information: The authors would like to thank past and present members of the laboratory for the development of methodology and helpful discussions, particularly Jessica Sullivan-Brown and Noriko Okabe. We would like to especially acknowledge Iain Drummond for his generosity in sharing protocols and suggestions; specifically his suggestions to improve our TEM fixation buffer and informing us of Point Grey{\textquoteright}s affordable high-speed cameras. We thank Shin-Yi Lin for critical reading of this chapter and Joe Goodhouse for help with confocal microscopy. Methods presented in this report were developed with funding to R.D.B. from the Edward Mallinckrodt Jr. Foundation, the New Jersey Commission on Cancer Research (04-2405-CCR-E0), the Polycystic Kidney Disease Foundation (117b2r), and from the National Institutes of Child Health and Human Development (1RO1HD048584). K.M.J. is supported by postdoctoral grant 0825952D from the American Heart Association. S.Y.T. runs the Lewis-Sigler Institute Imaging Facility, which is supported by grant P50GM071508 from the NIH/NIGMS.",
year = "2010",
doi = "10.1016/S0091-679X(10)97022-2",
language = "English (US)",
volume = "97",
pages = "415--435",
journal = "Methods in Cell Biology",
issn = "0091-679X",
publisher = "Academic Press Inc.",
number = "C",
}