Identification of a repeated sequence element required for efficient encapsidation of the adenovirus type 5 chromosome

P. Hearing, R. J. Samulski, W. L. Wishart, T. Shenk

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Adenovirus type 5 deletion mutants that lack portions of their cis-acting DNA encapsidation signal synthesized nearly normal levels of viral DNA and late polypeptides but failed to efficiently package the DNA into virus particles. A series of mutant viruses carrying small deletions were produced and used to identify a repeated element (AGTAAATTTGGGC and AGTAAGATTTGGCC) as a key component of the packaging signal. One copy of the repeat was sufficient to signal efficient packaging. The packaging domain could function near either end of the viral chromosome but was no longer active when moved several hundred base pairs toward the interior of the DNA molecule.

Original languageEnglish (US)
Pages (from-to)2555-2558
Number of pages4
JournalJournal of virology
Volume61
Issue number8
DOIs
StatePublished - 1987

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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