Identification of a possible nucleotide binding site in CheW, a protein required for sensory transduction in bacterial chemotaxis

A. Stock, J. Mottonen, T. Chen, J. Stock

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

CheW is an essential component of the system which mediates chemotaxis in Salmonella typhimurium and Escherichia coli. Here we report the nucleotide sequence of the cheW gene as well as the purification and characterization of the CheW protein. The DNA sequence predicts a protein of 18,000 molecular weight. The pure protein exhibits an apparent molecular weight of 18,000 during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Molecular sieve chromatography under nondenaturing conditions indicates a molecular weight of approximately 35,000, however. This result suggests that CheW is a homodimer. The predicted amino acid sequence between Thr-128 and Asp-160 fits a consensus exhibited by many proteins which bind purine nucleotides.

Original languageEnglish (US)
Pages (from-to)535-537
Number of pages3
JournalJournal of Biological Chemistry
Volume262
Issue number2
StatePublished - Jan 1 1987

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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