TY - JOUR
T1 - Human DNA sequences homologous to a protein coding region conserved between homeotic genes of Drosophila
AU - Levine, Michael
AU - Rubin, Gerald M.
AU - Tjian, Robert
N1 - Funding Information:
We thank Deborah Scott for performing the original screening of the human library during grad lab 201A. We are indebted to Tammy Learned for excellent technical assistance cfunng the sequencing of the homeo box region. Special thanks to Jim Merriweather, Jack Kirsch. and Bruce Malcolm for providing synthetic primers for sequencing DNA, and Christian Burke for computer analysis comparisons of the actin gene family and homeo box sequences homologies between human and Drosophila. Finally, we thank Tulle Hazelrigg, Bill Dynan, Suzi Mansour. and Ernst Hafen for critical reading of the manuscript. This work was funded by grants from the National Institutes of Health to R. T. and G. M. R. and partial support from a NIEHS center grant. M. S. L. was supported by a Jane Coffin Childs fellowship.
PY - 1984/10
Y1 - 1984/10
N2 - Several human DNA sequences were isolated by virtue of homology to a highly conserved region that has been identified in a number of homeotic genes in Drosophila. Structural analysis of the human DNAs indicate that two separate and distinct regions sharing a high degree of homology with the homeo box sequences of Drosophila are separated by only 5 kb in the human genome. Sequence determination of these regions reveals that both human DNA sequences contain a region capable of coding 61 amino acids, which shares greater than 90% homology with the peptide sequences specified by the homeo box domain of Drosophila homeotic genes, Antennapedia, fushi tarazu, and Ultrabithorax. By contrast, the human DNA sequences lying outside of the 190 nucleotide homeo box region share virtually no sequence homology, either with the flanking sequences of the other human clones or with flanking regions of the known Drosophila homeotic genes.
AB - Several human DNA sequences were isolated by virtue of homology to a highly conserved region that has been identified in a number of homeotic genes in Drosophila. Structural analysis of the human DNAs indicate that two separate and distinct regions sharing a high degree of homology with the homeo box sequences of Drosophila are separated by only 5 kb in the human genome. Sequence determination of these regions reveals that both human DNA sequences contain a region capable of coding 61 amino acids, which shares greater than 90% homology with the peptide sequences specified by the homeo box domain of Drosophila homeotic genes, Antennapedia, fushi tarazu, and Ultrabithorax. By contrast, the human DNA sequences lying outside of the 190 nucleotide homeo box region share virtually no sequence homology, either with the flanking sequences of the other human clones or with flanking regions of the known Drosophila homeotic genes.
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U2 - 10.1016/0092-8674(84)90261-7
DO - 10.1016/0092-8674(84)90261-7
M3 - Article
C2 - 6091895
AN - SCOPUS:0021717658
SN - 0092-8674
VL - 38
SP - 667
EP - 673
JO - Cell
JF - Cell
IS - 3
ER -