Human cytomegalovirus UL69 protein facilitates translation by associating with the mRNA cap-binding complex and excluding 4EBP1

Mariko Aoyagi, Miguel Gaspar, Thomas E. Shenk

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

4EBP1 is phosphorylated by the mTORC1 kinase. When mTORC1 activity is inhibited, hypophosphorylated 4EBP1 binds and sequesters eIF4E, a component of the mRNA cap-binding complex, and blocks translation.As a consequence,mTORC1 activity is needed tomaintain active translation. The human cytomegalovirus pUL38 protein preservesmTORC1 activity, keepingmost of the E4BP1 in the infectedcell in a hyperphosphorylated, inactive state. Here we report that a second viral protein, pUL69, also antagonizes the activity of 4EBP1, but by a separate mechanism. pUL69 interacts directly with eIF4A1, an element of thecap-bindingcomplex, andthepoly(A)-bindingprotein, which binds to the complex. When pUL69 accumulates during infection with wild-type virus, 4EBP1 is excluded from the complex. However, 4EBP1 is present in the cap-binding complex after infection with a pUL69-deficient virus, coincident with reduced accumulation of several late virus-coded proteins.We propose that pUL69 supports translation in human cytomegalovirus-infected cells by excluding hypophosphorylated 4EBP1 from the cap-binding complex.

Original languageEnglish (US)
Pages (from-to)2640-2645
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number6
DOIs
StatePublished - Feb 9 2010

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Eukaryotic initiation factor 4A
  • Poly(A)-binding protein
  • Translational control
  • Two-hybrid screen

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