TY - JOUR
T1 - How to Build a Bacterial Cell
T2 - MreB as the Foreman of E. coli Construction
AU - Shi, Handuo
AU - Bratton, Benjamin P.
AU - Gitai, Zemer
AU - Huang, Kerwyn Casey
N1 - Funding Information:
The authors thank the Huang, Gitai, and Shaevitz labs for useful discussions and Ethan Garner, Ariel Amir, Ned Wingreen, Spencer Cesar, and Carolina Tropini for critical readings of the manuscript. This work was supported by an Agilent Graduate Fellowship and a Stanford Interdisciplinary Graduate Fellowship (to H.S.), NIH Director’s New Innovator Award DP2OD006466 (to K.C.H.), NIH Director’s Pioneer Award DP1AI124669 (to Z.G.), NSF CAREER Award MCB-1149328 (to K.C.H.), NIH R01 GM107384 (to B.P.B. and Z.G.), the Stanford Systems Biology Center funded by NIH grant P50 GM107615 (to K.C.H.), the Glenn Foundation (to B.P.B.), and the Allen Discovery Center at Stanford on Systems Modeling of Infection (to K.C.H.). K.C.H. is a Chan Zuckerberg Biohub Investigator. This work was also supported in part by the National Science Foundation under grant PHYS-1066293 and the hospitality of the Aspen Center for Physics.
Funding Information:
The authors thank the Huang, Gitai, and Shaevitz labs for useful discussions and Ethan Garner, Ariel Amir, Ned Wingreen, Spencer Cesar, and Carolina Tropini for critical readings of the manuscript. This work was supported by an Agilent Graduate Fellowship and a Stanford Interdisciplinary Graduate Fellowship (to H.S.), NIH Director's New Innovator Award DP2OD006466 (to K.C.H.), NIH Director's Pioneer Award DP1AI124669 (to Z.G.), NSF CAREER Award MCB-1149328 (to K.C.H.), NIH R01 GM107384 (to B.P.B. and Z.G.), the Stanford Systems Biology Center funded by NIH grant P50 GM107615 (to K.C.H.), the Glenn Foundation (to B.P.B.), and the Allen Discovery Center at Stanford on Systems Modeling of Infection (to K.C.H.). K.C.H. is a Chan Zuckerberg Biohub Investigator. This work was also supported in part by the National Science Foundation under grant PHYS-1066293 and the hospitality of the Aspen Center for Physics.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/3/8
Y1 - 2018/3/8
N2 - Cell shape matters across the kingdoms of life, and cells have the remarkable capacity to define and maintain specific shapes and sizes. But how are the shapes of micron-sized cells determined from the coordinated activities of nanometer-sized proteins? Here, we review general principles that have surfaced through the study of rod-shaped bacterial growth. Imaging approaches have revealed that polymers of the actin homolog MreB play a central role. MreB both senses and changes cell shape, thereby generating a self-organizing feedback system for shape maintenance. At the molecular level, structural and computational studies indicate that MreB filaments exhibit tunable mechanical properties that explain their preference for certain geometries and orientations along the cylindrical cell body. We illustrate the regulatory landscape of rod-shape formation and the connectivity between cell shape, cell growth, and other aspects of cell physiology. These discoveries provide a framework for future investigations into the architecture and construction of microbes. How do microbes maintain their shape? This review takes a closer look at the role played by the actin homolog MreB in controlling rod-shaped bacterial growth.
AB - Cell shape matters across the kingdoms of life, and cells have the remarkable capacity to define and maintain specific shapes and sizes. But how are the shapes of micron-sized cells determined from the coordinated activities of nanometer-sized proteins? Here, we review general principles that have surfaced through the study of rod-shaped bacterial growth. Imaging approaches have revealed that polymers of the actin homolog MreB play a central role. MreB both senses and changes cell shape, thereby generating a self-organizing feedback system for shape maintenance. At the molecular level, structural and computational studies indicate that MreB filaments exhibit tunable mechanical properties that explain their preference for certain geometries and orientations along the cylindrical cell body. We illustrate the regulatory landscape of rod-shape formation and the connectivity between cell shape, cell growth, and other aspects of cell physiology. These discoveries provide a framework for future investigations into the architecture and construction of microbes. How do microbes maintain their shape? This review takes a closer look at the role played by the actin homolog MreB in controlling rod-shaped bacterial growth.
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U2 - 10.1016/j.cell.2018.02.050
DO - 10.1016/j.cell.2018.02.050
M3 - Review article
C2 - 29522748
AN - SCOPUS:85043322270
SN - 0092-8674
VL - 172
SP - 1294
EP - 1305
JO - Cell
JF - Cell
IS - 6
ER -