How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells

Jun Seok Oh, Xanthe Strudwick, Robert D. Short, Kotaro Ogawa, Akimitsu Hatta, Hiroshi Furuta, Nishtha Gaur, Sung Ha Hong, Allison J. Cowin, Hideo Fukuhara, Keiji Inoue, Masafumi Ito, Christine Charles, Roderick W. Boswell, James W. Bradley, David B. Graves, Endre J. Szili

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as "sparging." He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.

Original languageEnglish (US)
Article number203701
JournalApplied Physics Letters
Volume109
Issue number20
DOIs
StatePublished - Nov 14 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Fingerprint

Dive into the research topics of 'How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells'. Together they form a unique fingerprint.

Cite this