High-throughput end-on imaging of drosophila embryo for quantitative analysis of morphogens and signaling

Kwanghun Chung, Yoosik Kim, Emily Gong, Stanislav Yefimovic Shvartsman, Hang Lu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hydrodynamic forces due to the anisotropic shape of Drosophila embryo can rapidly orient it in an upright position. We used this effect and inertia hydrodynamics to design a microfluidics embryo trap array (META) that orders and vertically orients hundreds of embryos in a matter of minutes. Using META, we quantified morphogen gradients in the dorsoventral patterning system, a task that has been until now complicated by the need to manually orient individual embryos.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1676-1678
Number of pages3
StatePublished - Dec 1 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Keywords

  • Drosophila
  • High-throughput
  • Imaging
  • Morphogen gradient
  • Quantitative analysis
  • Systems biology

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