High-throughput approaches to profile RNA-protein interactions

Misha Nechay, Ralph E. Kleiner

Research output: Contribution to journalReview articlepeer-review

31 Scopus citations


RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalCurrent Opinion in Chemical Biology
StatePublished - Feb 2020

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry


  • CLIP
  • Proteomics
  • Ribonomics
  • Transcriptomics


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