Abstract
RNA-protein interactions play a critical role in post-transcriptional gene regulation. Characterizing these interactions in their native context has been challenging; however, advances in RNA sequencing and mass spectrometrybased proteomics combined with innovative chemical biological tools have heralded the development of robust strategies for performing biochemistry on a cellular scale. Herein, we review recent advances in the development and application of proteomic and transcriptomic approaches to profile cellular RNA-protein interactions, focusing on sequencing-based strategies and proteomic analysis of RNA-binding proteins, as well as approaches to address the role of RNA modifications in protein-RNA binding events.
Original language | English (US) |
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Pages (from-to) | 37-44 |
Number of pages | 8 |
Journal | Current Opinion in Chemical Biology |
Volume | 54 |
DOIs | |
State | Published - Feb 2020 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
Keywords
- CLIP
- Proteomics
- Ribonomics
- Transcriptomics