Herpes simplex virus type 1 restriction fragment polymorphism determined using southern hybridization

K. Umene, T. Eto, R. Mori, Y. Takagi, Lynn W. Enquist

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Regions of herpes simplex virus type 1 (HSV-1) DNA with variation in the size of restriction endonuclease fragments were identified by comparison of the Bam HI, KpnI or SalI restriction endonuclease digestion patterns among 15 HSV-1 isolates after hybridization with specific32P-labeled cloned HSV-1 DNA fragments. Of the types of restriction fragment polymorphism identified, one was a strain with a distinctly different restriction fragment than the prototype (loss or gain of restriction sites). Another type, the specific fragment varied only in size among strains. Thirteen distinct variations were identified. Ten were mapped to the unique sequence of the L component; two to the inverted repeat of the L component and one to the inverted repeat of the S component. The presence of a common ancestor from which some isolates of HSV-1 might derive was deduced from an analysis of the distribution of the thirteen variations among the 15 HSV-1 isolates.

Original languageEnglish (US)
Pages (from-to)275-290
Number of pages16
JournalArchives of Virology
Volume80
Issue number4
DOIs
StatePublished - Dec 1 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

Fingerprint Dive into the research topics of 'Herpes simplex virus type 1 restriction fragment polymorphism determined using southern hybridization'. Together they form a unique fingerprint.

  • Cite this