Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Roger J. Watson; John H. Weis; John S. Salstrom; Lynn W. Enquist

doi:10.1126/science.6289440

Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Roger J. Watson
, John H. Weis
, John S. Salstrom
, Lynn W. Enquist

Research output: Contribution to journal › Article › peer-review

165 Scopus citations

Abstract

The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.

Original language	English (US)
Pages (from-to)	381-384
Number of pages	4
Journal	Science
Volume	218
Issue number	4570
DOIs	https://doi.org/10.1126/science.6289440
State	Published - 1982

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title = "Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli",

abstract = "The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.",

author = "Watson, \{Roger J.\} and Weis, \{John H.\} and Salstrom, \{John S.\} and Enquist, \{Lynn W.\}",

year = "1982",

doi = "10.1126/science.6289440",

language = "English (US)",

volume = "218",

pages = "381--384",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4570",

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T1 - Herpes simplex virus type-1 glycoprotein D gene

T2 - Nucleotide sequence and expression in Escherichia coli

AU - Watson, Roger J.

AU - Weis, John H.

AU - Salstrom, John S.

AU - Enquist, Lynn W.

PY - 1982

Y1 - 1982

N2 - The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.

AB - The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.

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UR - https://www.scopus.com/pages/publications/0019943323#tab=citedBy

U2 - 10.1126/science.6289440

DO - 10.1126/science.6289440

M3 - Article

C2 - 6289440

AN - SCOPUS:0019943323

SN - 0036-8075

VL - 218

SP - 381

EP - 384

JO - Science

JF - Science

IS - 4570

ER -

Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Abstract

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