Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Roger J. Watson; John H. Weis; John S. Salstrom; Lynn W. Enquist

doi:10.1126/science.6289440

Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Roger J. Watson, John H. Weis, John S. Salstrom, Lynn W. Enquist

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164 Scopus citations

Abstract

The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.

Original language	English (US)
Pages (from-to)	381-384
Number of pages	4
Journal	Science
Volume	218
Issue number	4570
DOIs	https://doi.org/10.1126/science.6289440
State	Published - 1982

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abstract = "The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.",

author = "Watson, {Roger J.} and Weis, {John H.} and Salstrom, {John S.} and Enquist, {Lynn W.}",

year = "1982",

doi = "10.1126/science.6289440",

language = "English (US)",

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pages = "381--384",

journal = "Science",

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T1 - Herpes simplex virus type-1 glycoprotein D gene

T2 - Nucleotide sequence and expression in Escherichia coli

AU - Watson, Roger J.

AU - Weis, John H.

AU - Salstrom, John S.

AU - Enquist, Lynn W.

PY - 1982

Y1 - 1982

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AB - The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.

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VL - 218

SP - 381

EP - 384

JO - Science

JF - Science

IS - 4570

ER -

Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Abstract

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