Herpes simplex virus type-1 glycoprotein D gene: Nucleotide sequence and expression in Escherichia coli

Roger J. Watson, John H. Weis, John S. Salstrom, Lynn W. Enquist

Research output: Contribution to journalArticle

145 Scopus citations

Abstract

The protein coding region ofthe herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.

Original languageEnglish (US)
Pages (from-to)381-384
Number of pages4
JournalScience
Volume218
Issue number4570
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

All Science Journal Classification (ASJC) codes

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