H/ACA snRNP–dependent ribosome biogenesis regulates translation of polyglutamine proteins

Shane M. Breznak, Yingshi Peng, Limin Deng, Noor M. Kotb, Zachary Flamholz, Ian T. Rapisarda, Elliot T. Martin, Kara A. LaBarge, Dan Fabris, Elizabeth R. Gavis, Prashanth Rangan

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Stem cells in many systems, including Drosophila germline stem cells (GSCs), increase ribosome biogenesis and translation during terminal differentiation. Here, we show that the H/ACA small nuclear ribonucleoprotein (snRNP) complex that promotes pseudouridylation of ribosomal RNA (rRNA) and ribosome biogenesis is required for oocyte specification. Reducing ribosome levels during differentiation decreased the translation of a subset of messenger RNAs that are enriched for CAG trinucleotide repeats and encode polyglutamine-containing proteins, including differentiation factors such as RNA-binding Fox protein 1. Moreover, ribosomes were enriched at CAG repeats within transcripts during oogenesis. Increasing target of rapamycin (TOR) activity to elevate ribosome levels in H/ACA snRNP complex–depleted germlines suppressed the GSC differentiation defects, whereas germlines treated with the TOR inhibitor rapamycin had reduced levels of polyglutamine-containing proteins. Thus, ribosome biogenesis and ribosome levels can control stem cell differentiation via selective translation of CAG repeat–containing transcripts.

Original languageEnglish (US)
Article numbereade5492
JournalScience Advances
Issue number25
StatePublished - Jun 2023

All Science Journal Classification (ASJC) codes

  • General


Dive into the research topics of 'H/ACA snRNP–dependent ribosome biogenesis regulates translation of polyglutamine proteins'. Together they form a unique fingerprint.

Cite this